Abstract | BACKGROUND: METHODS: RESULTS: The expression rate of MTA2 in gastric cancer tissues was 55.9% (71/127), and its expression was closely related to the depth of tumor invasion, lymph nodes metastasis, and TNM staging. MTA2 knockdown in human SGC-7901 and AGS gastric cancer cells significantly inhibited migration and invasion in vitro, and disrupted structure of cytoskeleton. MTA2 knockdown also attenuated xenografts growth and lung metastasis in nude mice model. MTA2 expression was positively correlated with transcription factor Sp1 in gastric cancer tissues (r = 0.326, P < 0.001). Sp1 bound to human MTA2 gene promoter at region from -1043 bp to -843 bp. Transcriptional activity of MTA2 promoter could be enhanced by Sp1 overexpression. CONCLUSIONS: MTA2 knockdown impairs invasion and metastasis of gastric cancer cells, and attenuates xenografts growth in vivo. Sp1 regulates MTA2 expression at transcriptional level.
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Authors | Chenfei Zhou, Jun Ji, Qu Cai, Min Shi, Xuehua Chen, Yingyan Yu, Bingya Liu, Zhenggang Zhu, Jun Zhang |
Journal | Molecular cancer
(Mol Cancer)
Vol. 12
Issue 1
Pg. 102
(Sep 08 2013)
ISSN: 1476-4598 [Electronic] England |
PMID | 24010737
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Repressor Proteins
- Sp1 Transcription Factor
- MTA2 protein, human
- Histone Deacetylases
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Topics |
- Actin Cytoskeleton
(metabolism)
- Adenocarcinoma
(metabolism, mortality, secondary)
- Animals
- Binding Sites
- Cell Line, Tumor
- Cell Movement
- Gene Expression Regulation, Neoplastic
- Histone Deacetylases
(genetics, metabolism)
- Humans
- Lung Neoplasms
(genetics, metabolism, secondary)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Invasiveness
- Neoplasm Transplantation
- Promoter Regions, Genetic
- Repressor Proteins
(genetics, metabolism)
- Sp1 Transcription Factor
(physiology)
- Stomach Neoplasms
(genetics, metabolism, mortality, pathology)
- Transcription, Genetic
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