Abstract | BACKGROUND: METHODS: Real-time PCR and Western blot were employed to detect the expression of QSOX1 in human pancreatic cancer cell lines under hypoxic condition. Luciferase reporter and ChIP assays were used to assess the regulation of QSOX1 by hypoxia-inducible factor 1 (HIF-1). Small interfering RNA ( siRNA) was applied to knock down endogenous expression of QSOX1. Matrigel-coated invasion chamber essays were conducted to detect the invasion capacity of QSOX1-depleted cells. RESULTS: Both hypoxia and hypoxia mimicking reagent up-regulated the expression of QSOX1 in human pancreatic cancer cell lines. Knockdown of HIF-1α eliminated hypoxia induced QSOX1 expression. HIF-1α was found directly bound to two hypoxia-response elements (HRE) of QSOX1 gene, both of which were required for HIF-1 induced QSOX1 expression. Moreover, QSOX1 silencing blocked hypoxia-induced pancreatic cancer cells invasion. CONCLUSION:
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Authors | Chen-Ye Shi, Yue Fan, Bin Liu, Wen-Hui Lou |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 32
Issue 3
Pg. 561-8
( 2013)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 24008827
(Publication Type: Journal Article)
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Copyright | © 2013 S. Karger AG, Basel. |
Chemical References |
- Hypoxia-Inducible Factor 1, alpha Subunit
- RNA, Messenger
- RNA, Small Interfering
- Oxidoreductases Acting on Sulfur Group Donors
- QSOX1 protein, human
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 9
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Topics |
- Cell Hypoxia
- Cell Line, Tumor
- Cell Movement
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
(antagonists & inhibitors, genetics, metabolism)
- Matrix Metalloproteinase 2
(metabolism)
- Matrix Metalloproteinase 9
(metabolism)
- Oxidoreductases Acting on Sulfur Group Donors
(antagonists & inhibitors, genetics, metabolism)
- Pancreatic Neoplasms
- Protein Binding
- RNA Interference
- RNA, Messenger
(metabolism)
- RNA, Small Interfering
(metabolism)
- Response Elements
- Up-Regulation
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