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The role of potassium channel in silica nanoparticle-induced inflammatory effect in human vascular endothelial cells in vitro.

Abstract
Exposure to nanoparticles became popular in industry and daily life. Nano-SiO₂ was shown to have an adverse effect to vascular endothelial cell although the mechanisms remain unclear. To test whether the nano-SiO₂'s harmful effect was related to the potassium channel, human umbilical vascular endothelial cells (HUVECs) were treated with nano-SiO₂ in different dose. Cell survival rate and lactate dehydrogenase (LDH) as cytotoxic parameters, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) as inflammation indicators were determined. The electrophysiological changes and function of potassium channel were detected with patch clamp and channel blockers. It was found that nano-SiO₂ exposure decreased cell survival rate, increased LDH leakage, TNF-α and IL-6 production. The potassium channel activity was increased in the opening rate and current intensity. Furthermore, potassium channel blockers tetraethylammonium (TEA), 4-amino pyridine (4-AP), and margatoxin (MGTX) reduced the nano-SiO₂-induced cytotoxity and inflammation, i.e., increase in the cell survival rate, and decrease in the LDH leakage and production of TNF-α and IL-6. It might be concluded that the nano-SiO₂-induced inflammation and cytotoxicity at HUVECs was associated with the activation of potassium channel.
AuthorsLi Yang, Qinqian Yan, Jing Zhao, Jun Li, Xuecong Zong, Lei Yang, Zhenglun Wang
JournalToxicology letters (Toxicol Lett) Vol. 223 Issue 1 Pg. 16-24 (Oct 23 2013) ISSN: 1879-3169 [Electronic] Netherlands
PMID24001805 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Chemical References
  • Interleukin-6
  • Potassium Channel Blockers
  • Potassium Channels
  • Tumor Necrosis Factor-alpha
  • Silicon Dioxide
  • L-Lactate Dehydrogenase
Topics
  • Cell Survival (drug effects)
  • Endothelium, Vascular (cytology, drug effects, metabolism)
  • Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
  • Humans
  • Interleukin-6 (metabolism)
  • L-Lactate Dehydrogenase (metabolism)
  • Microscopy, Electron, Transmission
  • Microscopy, Fluorescence
  • Nanoparticles (toxicity)
  • Patch-Clamp Techniques
  • Potassium Channel Blockers (pharmacology)
  • Potassium Channels (metabolism)
  • Silicon Dioxide (chemistry, toxicity)
  • Tumor Necrosis Factor-alpha (metabolism)
  • Vasculitis (chemically induced, metabolism)

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