Abstract |
Exposure to nanoparticles became popular in industry and daily life. Nano-SiO₂ was shown to have an adverse effect to vascular endothelial cell although the mechanisms remain unclear. To test whether the nano-SiO₂'s harmful effect was related to the potassium channel, human umbilical vascular endothelial cells (HUVECs) were treated with nano-SiO₂ in different dose. Cell survival rate and lactate dehydrogenase (LDH) as cytotoxic parameters, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) as inflammation indicators were determined. The electrophysiological changes and function of potassium channel were detected with patch clamp and channel blockers. It was found that nano-SiO₂ exposure decreased cell survival rate, increased LDH leakage, TNF-α and IL-6 production. The potassium channel activity was increased in the opening rate and current intensity. Furthermore, potassium channel blockers tetraethylammonium ( TEA), 4-amino pyridine (4-AP), and margatoxin ( MGTX) reduced the nano-SiO₂-induced cytotoxity and inflammation, i.e., increase in the cell survival rate, and decrease in the LDH leakage and production of TNF-α and IL-6. It might be concluded that the nano-SiO₂-induced inflammation and cytotoxicity at HUVECs was associated with the activation of potassium channel.
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Authors | Li Yang, Qinqian Yan, Jing Zhao, Jun Li, Xuecong Zong, Lei Yang, Zhenglun Wang |
Journal | Toxicology letters
(Toxicol Lett)
Vol. 223
Issue 1
Pg. 16-24
(Oct 23 2013)
ISSN: 1879-3169 [Electronic] Netherlands |
PMID | 24001805
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Interleukin-6
- Potassium Channel Blockers
- Potassium Channels
- Tumor Necrosis Factor-alpha
- Silicon Dioxide
- L-Lactate Dehydrogenase
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Topics |
- Cell Survival
(drug effects)
- Endothelium, Vascular
(cytology, drug effects, metabolism)
- Human Umbilical Vein Endothelial Cells
(drug effects, metabolism)
- Humans
- Interleukin-6
(metabolism)
- L-Lactate Dehydrogenase
(metabolism)
- Microscopy, Electron, Transmission
- Microscopy, Fluorescence
- Nanoparticles
(toxicity)
- Patch-Clamp Techniques
- Potassium Channel Blockers
(pharmacology)
- Potassium Channels
(metabolism)
- Silicon Dioxide
(chemistry, toxicity)
- Tumor Necrosis Factor-alpha
(metabolism)
- Vasculitis
(chemically induced, metabolism)
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