Abstract | BACKGROUND: Previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. In the current study, effects of IL-4 against morphological changes and tissue degradation induced by IL-1α on bovine nasal cartilage (BNC) explants were investigated. METHODS: Fresh BNC samples were prepared from a slaughterhouse under sterile conditions. BNC explants culture was treated with both IL-lα (10 ng/ml) and IL-4 (50 ng/ml) at the same time for 28 days. The morphological characteristics of explants were assessed by using histology techniques and invert microscopy. Matrix metalloproteinase-1 (MMP-1) production was assessed within different days by using Western blotting. RESULTS: IL-lα induced prominent cartilage morphology degradation. The pro and active form of MMP-1 band substantially increased at day 21 of culture. In the presence of both IL-lα and IL-4, chondrocytes preserved their ordinary normal phenotype with intact extracellular matrix. In addition, a significant reduction in pro-MMP-1and inhibition of active MMP-1 was seen. CONCLUSION: In conclusion, IL-4 could be regarded as a potential candidate in cartilage protecting against the degradation changes of IL-lα. It seems that the preservation effect of IL-4 is associated with significant reduction of MMP-1.
|
Authors | Maryam Yadegari, Mahmoud Orazizadeh, Mahmoud Hashemitabar, Ali Khodadadi |
Journal | Iranian biomedical journal
(Iran Biomed J)
Vol. 17
Issue 4
Pg. 187-93
( 2013)
ISSN: 2008-823X [Electronic] Iran |
PMID | 23999714
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Culture Media
- Interleukin-1alpha
- Protective Agents
- Proteoglycans
- Interleukin-4
- Matrix Metalloproteinase 1
|
Topics |
- Animals
- Blotting, Western
- Cattle
- Cell Shape
- Chondrocytes
(drug effects, enzymology, pathology)
- Culture Media
(pharmacology)
- Extracellular Matrix
(drug effects, metabolism)
- Interleukin-1alpha
(pharmacology)
- Interleukin-4
(pharmacology)
- Matrix Metalloproteinase 1
(metabolism)
- Nose
(pathology)
- Protective Agents
(pharmacology)
- Proteoglycans
(metabolism)
|