Characterization of 2-(methylamino)alkanoic acid capacity to restrict blood-brain phenylalanine transport in Pah enu2 mice: preliminary findings.

Abstract

BACKGROUND:

Our laboratory seeks a pharmacotherapeutic intervention for PKU that utilizes non-physiological amino acids (NPAAs) to block the accumulation of phenylalanine (Phe) in the brain. In previous studies (Vogel et al. 2013), methylation of the amino group of 2-aminoisobutyrate (AIB) provided an enhanced degree of selectivity for Phe restriction into the brain of Pah(enu2) mice in comparison to unmethylated AIB, leading to the hypothesis that 2-(methylamino)alkanoic acid analogs of AIB might represent targeted inhibitors of Phe accretion into the brain.

METHODS:

Pah(enu2) and control mice were intraperitoneally administered (500-750 mg/kg body weight, once daily; standard 19% protein diet) AIB, methyl AIB (MAIB), isovaline, and two MAIB analogs, 2-methyl-2-(methylamino)butanoic (MeVal) and 3-methyl-2-(methylamino)pentanoic (MePent) acids for one week, followed by brain and blood isolation for amino acid analyses using UPLC.

RESULTS:

In the brain, AIB significantly reduced Phe accretion in Pah(enu2) mice, while MeVal significantly improved glutamine and aspartic acids. Four of five test compounds improved brain threonine and arginine levels. AIB, MAIB and IsoVal significantly reduced blood Phe, with no effect of any drug intervention on other sera amino acids.

CONCLUSIONS:

Further evaluation of AIB and the 2-(methylamino)alkanoic acids as inhibitors of brain Phe accumulation in Pah(enu2) mice is warranted, with more detailed evaluations of route of administration, combinatorial intervention, and detailed toxicity studies.

• Authors: Kara R Vogel, Erland Arning, Brandi L Wasek, Teodoro Bottiglieri, K Michael Gibson
• Journal: Molecular genetics and metabolism (Mol Genet Metab) Vol. 110 Suppl Pg. S71-8 ( 2013) ISSN: 1096-7206 [Electronic] United States
• PMID: 23999161 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2013.

Chemical References

• 3-methyl-2-(methylamino)pentanoic acid
• Acids, Acyclic
• Aminoisobutyric Acids
• Large Neutral Amino Acid-Transporter 1
• N-methylvaline
• Isoleucine
• 2-aminoisobutyric acid
• 2-(methylamino)isobutyric acid
• Phenylalanine
• isovaline
• Valine

Topics

• Acids, Acyclic (administration & dosage, pharmacology)
• Aminoisobutyric Acids (administration & dosage, pharmacology)
• Animals
• Blood-Brain Barrier (metabolism)
• Brain (metabolism)
• Disease Models, Animal
• Humans
• Isoleucine (administration & dosage, analogs & derivatives, pharmacology)
• Large Neutral Amino Acid-Transporter 1 (chemistry, metabolism)
• Methylation
• Mice
• Mice, Transgenic
• Molecular Targeted Therapy
• Organ Specificity
• Phenylalanine (blood, metabolism)
• Phenylketonurias (drug therapy)
• Protein Conformation
• Protein Folding
• Valine (administration & dosage, analogs & derivatives, pharmacology)