Different effects of Alzheimer's peptide Aβ(1-40) oligomers and fibrils on supported lipid membranes.

Abstract

Beta-amyloid (1-40) is one of the two most abundant species of amyloid-beta peptides present as fibrils in the extracellular senile plaques in the brain of Alzheimer's patients. Recently, the molecular aggregates constituting the early stage of fibril formation, i.e., oligomers and protofibrils, have been investigated as the main responsible for amyloid-beta cytotoxic effect. The molecular mechanism leading to neurodegeneration is still under debate, and it is common opinion that it may reside in the interaction between amyloid species and the neural membrane. In this investigation Atomic Force Microscopy and spectroscopy have been used to understand how structural (and mechanical) properties of POPC/POPS lipid bilayers, simulating the phospholipid composition and negative net charge of neuritic cell membranes, are influenced by the interaction with Aβ(1-40), in different stages of the peptide aggregation. Substantial differences in the damage caused to the lipid bilayers have been observed, confirming the toxic effect exerted especially by Aβ(1-40) prefibrillar oligomers.

• Authors: Claudio Canale, Silvia Seghezza, Silvia Vilasi, Rita Carrotta, Donatella Bulone, Alberto Diaspro, Pier Luigi San Biagio, Silvia Dante
• Journal: Biophysical chemistry (Biophys Chem) Vol. 182 Pg. 23-9 (Dec 01 2013) ISSN: 1873-4200 [Electronic] Netherlands
• PMID: 23998637 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Lipid Bilayers
• Peptide Fragments
• Phosphatidylcholines
• Phosphatidylserines
• amyloid beta-protein (1-40)
• 1-palmitoyl-2-oleoylglycero-3-phosphoserine
• 1-palmitoyl-2-oleoylphosphatidylcholine

Topics

• Alzheimer Disease (metabolism, pathology)
• Amyloid beta-Peptides (chemistry, metabolism, toxicity)
• Cell Membrane (chemistry, metabolism)
• Humans
• Lipid Bilayers (chemistry, metabolism)
• Microscopy, Atomic Force
• Neurons (drug effects, metabolism)
• Peptide Fragments (chemistry, metabolism, toxicity)
• Phosphatidylcholines (chemistry)
• Phosphatidylserines (chemistry)