Abstract | OBJECTIVE: To evaluate antioxidant, anti-inflammatory, hepatoprotective and vasorelaxant activities of Populus nigra flower buds ethanolic extract. METHODS:
Antioxidant and anti-inflammatory activities of the extract were assessed using respectively the ABTS test and the animal model of carrageenan-induced paw edema. Protection from hepatic toxicity caused by aluminum was examined by histopathologic analysis of liver sections. Vasorelaxant effect was estimated in endothelium-intact and -rubbed rings of porcine coronary arteries precontracted with high concentration of U46619. RESULTS: The results showed a moderate antioxidant activity (40%), but potent anti-inflammatory activity (49.9%) on carrageenan-induced mice paw edema, and also as revealed by histopathologic examination, complete protection against AlCl₃-induced hepatic toxicity. Relaxant effects of the same extract on vascular preparation from porcine aorta precontracted with high concentration of U46619 were considerable at 10⁻¹ g/L, and comparable (P>0.05) between endothelium-intact (67.74%, IC₅₀=0.04 mg/mL) and -rubbed (72.72%, IC₅₀=0.075 mg/mL) aortic rings. CONCLUSIONS: The extract exerted significant anti-inflammatory, hepatoprotective and vasorelaxant activities, the latter being endothelium-independent believed to be mediated mainly by the ability of components present in the extract to exert antioxidant properties, probably related to an inhibition of Ca²⁺ influx.
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Authors | Nadjet Debbache-Benaida, Dina Atmani-Kilani, Valérie Barbara Schini-Keirth, Nouredine Djebbli, Djebbar Atmani |
Journal | Asian Pacific journal of tropical biomedicine
(Asian Pac J Trop Biomed)
Vol. 3
Issue 9
Pg. 697-704
(Sep 2013)
ISSN: 2221-1691 [Print] China |
PMID | 23998009
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aluminum Compounds
- Anti-Inflammatory Agents
- Cardiovascular Agents
- Chlorides
- Flavonoids
- Phenols
- Plant Extracts
- Protective Agents
- Tannins
- Nitric Oxide
- Aluminum Chloride
- Carrageenan
- Nitric Oxide Synthase Type III
- Nos3 protein, mouse
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Topics |
- Aluminum Chloride
- Aluminum Compounds
(toxicity)
- Analysis of Variance
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Cardiovascular Agents
(pharmacology)
- Carrageenan
(toxicity)
- Chemical and Drug Induced Liver Injury
(drug therapy, pathology)
- Chlorides
(toxicity)
- Edema
(chemically induced, drug therapy, physiopathology)
- Female
- Flavonoids
(analysis)
- Flowers
(chemistry)
- Liver
(drug effects, pathology)
- Male
- Mice
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type III
(metabolism)
- Phenols
(analysis)
- Plant Extracts
(chemistry, pharmacology)
- Populus
(chemistry)
- Protective Agents
(pharmacology)
- Tannins
(analysis)
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