Protective effect of N-acetylserotonin against acute hepatic ischemia-reperfusion injury in mice.

Abstract

The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.

• Authors: Shuna Yu, Jie Zheng, Zhengchen Jiang, Caixing Shi, Jin Li, Xiaodong Du, Hailiang Wang, Jiying Jiang, Xin Wang
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 14 Issue 9 Pg. 17680-93 (Aug 29 2013) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 23994834 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Serotonin
• Malondialdehyde
• Superoxide Dismutase
• Aspartate Aminotransferases
• Caspase 3
• N-acetylserotonin

Topics

• Animals
• Apoptosis (drug effects)
• Aspartate Aminotransferases (blood)
• Blotting, Western
• Caspase 3 (metabolism)
• Immunohistochemistry
• In Situ Nick-End Labeling
• Liver (drug effects, injuries, metabolism)
• Male
• Malondialdehyde (blood)
• Mice
• Reperfusion Injury (drug therapy, metabolism)
• Serotonin (analogs & derivatives, therapeutic use)
• Superoxide Dismutase (blood)