Stabilization of tooth movement by administration of reveromycin A to osteoprotegerin-deficient knockout mice.

Abstract

INTRODUCTION:

In this study, mechanical stress in the form of tooth movement was applied to osteoprotegerin-deficient knockout mice, which served as an animal model for juvenile Paget's disease. To compare and evaluate bone turnover and response of the surrounding bony tissue, we administered reveromycin A. We also investigated the ability of reveromycin A to control osteoclastic activity in juvenile Paget's disease.

METHODS:

Eight-week-old male osteoprotegerin-deficient knockout and wild-type mice were injected with reveromycin A (15 mg/kg of body weight) intraperitoneally twice daily. An elastic module was inserted interproximally between the maxillary left first and second molars.

RESULTS:

Administration of reveromycin A to osteoprotegerin-deficient knockout mice reduced tooth movement distances, increased bone volumes at the interradicular septum, decreased osteoclast counts, and reduced serum alkaline phosphatase and tartrate resistant acid phosphatase. Reveromycin A administration also caused a temporal shift in peak Runx2 staining in osteoprotegerin-deficient knockout mice so that the overall staining time course was similar to that observed for wild-type mice.

CONCLUSIONS:

Reveromycin A administration in osteoprotegerin-deficient knockout mice inhibited bone resorption and normalized bone formation. As a result, normal bone turnover was obtained.

• Authors: Takahiro Yabumoto, Ken Miyazawa, Masako Tabuchi, Satsuki Shoji, Miyuki Tanaka, Manami Kadota, Mamoru Yoshizako, Makoto Kawatani, Hiroyuki Osada, Hatsuhiko Maeda, Shigemi Goto
• Journal: American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics (Am J Orthod Dentofacial Orthop) Vol. 144 Issue 3 Pg. 368-80 (Sep 2013) ISSN: 1097-6752 [Electronic] United States
• PMID: 23992809 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

Chemical References

• Antimitotic Agents
• Core Binding Factor Alpha 1 Subunit
• Osteoprotegerin
• Pyrans
• Spiro Compounds
• reveromycin A

Topics

• Alveolar Bone Loss (prevention & control)
• Animals
• Antimitotic Agents (administration & dosage, pharmacology, therapeutic use)
• Bone Remodeling (drug effects)
• Core Binding Factor Alpha 1 Subunit
• Dental Stress Analysis
• Disease Models, Animal
• Injections, Intraperitoneal
• Male
• Mice
• Mice, Knockout
• Osteitis Deformans (drug therapy)
• Osteoprotegerin (deficiency)
• Pyrans (administration & dosage, pharmacology, therapeutic use)
• Secondary Prevention
• Spiro Compounds (administration & dosage, pharmacology, therapeutic use)
• Tooth Movement Techniques