Immunohistochemical detection of receptor activator nuclear κB ligand and osteoprotegerin in odontogenic cysts and tumors.

Abstract

PURPOSE:

The aim of the present study was to compare the immunohistochemical detection of receptor activator nuclear κB ligand (RANKL) and osteoprotegerin (OPG) in radicular cysts (RCs), dentigerous cysts (DCs), solid ameloblastomas (SAs), and keratocystic odontogenic tumors (KOTs).

MATERIALS AND METHODS:

A total of 20 RCs, 20 DCs, 20 KOTs, 14 dental follicles (DFs), and 18 SAs were evaluated by immunohistochemistry using anti-RANKL and anti-OPG antibodies. The analysis was quantitative, and the number of positive cells was counted in 10 microscopic high-power fields (400×).

RESULTS:

The DFs, KOTs, and SAs showed higher expression of RANKL than did the RCs and DCs in the epithelium (P < .05). The epithelial expression of OPG was higher in the DFs, KOTs, RCs, and DCs than in the SAs (P < .05). The ratio of OPG less than RANKL was more frequent in SAs and OPG greater than RANKL in DCs (P < .05).

CONCLUSIONS:

Our results have shown differences in RANKL and OPG detection in the odontogenic cysts and tumors studied. The higher RANKL and lower OPG detection in SA could play a role in bone resorption, compatible with the tumor's biologic behavior.

• Authors: Felipe Rodrigues de Matos, Maiara de Moraes, Emília Beatriz das Neves Silva, Hébel Cavalcanti Galvão, Roseana de Almeida Freitas
• Journal: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (J Oral Maxillofac Surg) Vol. 71 Issue 11 Pg. 1886-92 (Nov 2013) ISSN: 1531-5053 [Electronic] United States
• PMID: 23992784 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2013 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers, Tumor
• Coloring Agents
• Osteoprotegerin
• RANK Ligand
• TNFRSF11B protein, human
• TNFSF11 protein, human

Topics

• Ameloblastoma (pathology)
• Biomarkers, Tumor (analysis)
• Cell Count
• Coloring Agents
• Dental Sac (pathology)
• Dentigerous Cyst (pathology)
• Epithelial Cells (pathology)
• Humans
• Immunohistochemistry
• Odontogenic Cysts (pathology)
• Odontogenic Tumors (pathology)
• Osteoprotegerin (analysis)
• RANK Ligand (analysis)
• Radicular Cyst (pathology)
• Stromal Cells