Oral administration of benzyl-isothiocyanate inhibits in vivo growth of subcutaneous xenograft tumors of human malignant melanoma A375.S2 cells.

Abstract

A number of experiments have demonstrated that benzyl-isothiocyanate (BITC) induces cytotoxic cell death through the induction of apoptosis in various human cancer cell lines. In the present study, we investigated the effects of BITC on the growth of A375.S2 cell xenograft tumors in nude BALB/c mice in vivo. The A375.S2 cancer cells were inoculated subcutaneously into the lower flanks of each nude mouse. After cancer cell inoculation, all animals were maintained in the animal room for seven days and all mice produced one palpable tumor. Animals were randomly divided into two groups, each mouse was individually given intraperitoneal injections of BITC (20 mg/kg) or not (control). Results from the in vivo experiments indicated that BITC did not significantly affect the body weight of nude BALB/c mice bearing xenograft A375.S2 cell tumors but did significantly decrease the tumor weight.

• Authors: Wei-Ya Ni, Yu-Ping Hsiao, Shu-Chun Hsu, Shu-Ching Hsueh, Chuan-Hsun Chang, Bin-Chuan Ji, Jai-Sing Yang, Hsu-Feng Lu, Jing-Gung Chung
• Journal: In vivo (Athens, Greece) (In Vivo) 2013 Sep-Oct Vol. 27 Issue 5 Pg. 623-6 ISSN: 1791-7549 [Electronic] Greece
• PMID: 23988897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Isothiocyanates
• benzyl isothiocyanate

Topics

• Administration, Oral
• Animals
• Antineoplastic Agents (administration & dosage)
• Cell Line, Tumor
• Disease Models, Animal
• Humans
• Isothiocyanates (administration & dosage)
• Male
• Melanoma (drug therapy, pathology)
• Mice
• Tumor Burden (drug effects)
• Xenograft Model Antitumor Assays