HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Virus-like particles containing the tetrameric ectodomain of influenza matrix protein 2 and flagellin induce heterosubtypic protection in mice.

Abstract
The ectodomain of matrix protein 2 (M2e) is highly conserved among influenza A viruses and can be a promising candidate antigen for a broadly cross-protective vaccine. In this study, a tetrameric M2e (tM2e) and a truncated form of flagellin (tFliC) were coincorporated into virus-like particles (VLPs) to enhance its immunogenicity. Our data showed that the majority of M2e in VLPs was presented as tetramers by introducing a foreign tetramerization motif GCN4. Intranasal immunization with tM2e VLPs significantly enhanced the levels of serum IgG and IgG subclasses compared to soluble M2e (sM2e) in mice. tM2e VLPs also induced higher M2e-specific T-cell and mucosal antibody responses, conferring complete protection against homologous influenza virus infection. The immunogenicity of tM2e VLPs was further enhanced by coincorporation of the membrane-anchored tFliC (tM2e chimeric VLPs) or coadministration with tFliC VLPs as a mixture, but not the soluble flagellin, inducing strong humoral and cellular immune responses conferring cross-protection against lethal challenge with heterotypic influenza viruses. These results support the development of tM2e chimeric VLPs as universal vaccines and warrant further investigation.
AuthorsLi Wang, Ying-Chun Wang, Hao Feng, Tamanna Ahmed, Richard W Compans, Bao-Zhong Wang
JournalBioMed research international (Biomed Res Int) Vol. 2013 Pg. 686549 ( 2013) ISSN: 2314-6141 [Electronic] United States
PMID23984396 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Immunoglobulin G
  • M2 protein, Influenza A virus
  • Viral Matrix Proteins
  • Flagellin
Topics
  • Animals
  • Antibody Formation (immunology)
  • Cell Line
  • Cell Membrane (metabolism, ultrastructure)
  • Cross Reactions (immunology)
  • Dogs
  • Female
  • Flagellin (immunology)
  • Immunity, Humoral (immunology)
  • Immunity, Mucosal (immunology)
  • Immunoglobulin G (blood)
  • Influenza A virus (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections (blood, immunology, prevention & control, virology)
  • Protein Structure, Quaternary
  • Protein Structure, Tertiary
  • T-Lymphocytes (immunology)
  • Viral Matrix Proteins (chemistry, immunology)
  • Virion (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: