Synthesis and evaluation of mutual azo prodrug of 5-aminosalicylic acid linked to 2-phenylbenzoxazole-2-yl-5-acetic acid in ulcerative colitis.

Abstract

In this study, the syntheses of 4-aminophenylbenzoxazol-2-yl-5-acetic acid, (an analogue of a known nonsteroidal anti-inflammatory drug [NSAID]) and 5-[4-(benzoxazol-2-yl-5-acetic acid)phenylazo]-2-hydroxybenzoic acid (a novel mutual azo prodrug of 5-aminosalicylic acid [5-ASA]) are reported. The structures of the synthesized compounds were confirmed using infrared (IR), hydrogen-1 nuclear magnetic resonance (1H NMR), and mass spectrometry (MS) spectroscopy. Incubation of the azo compound with rat cecal contents demonstrated the susceptibility of the prepared azo prodrug to bacterial azoreductase enzyme. The azo compound and the 4-aminophenylbenzoxazol-2-yl-5-acetic acid were evaluated for inflammatory bowel diseases, in trinitrobenzenesulfonic acid (TNB)-induced colitis in rats. The synthesized diazo compound and the 4-aminophenylbenzoxazol-2-yl-5-acetic acid were found to be as effective as 5-aminosalicylic acid for ulcerative colitis. The results of this work suggest that the 4-aminophenylbenzoxazol-2-yl-5-acetic acid may represent a new lead for treatment of ulcerative colitis.

• Authors: Jamal A Jilani, Maha Shomaf, Karem H Alzoubi
• Journal: Drug design, development and therapy (Drug Des Devel Ther) Vol. 7 Pg. 691-8 ( 2013) ISSN: 1177-8881 [Electronic] New Zealand
• PMID: 23983456 (Publication Type: Comparative Study, Journal Article)

Chemical References

• 4-aminophenylbenzoxazol-2-yl-5-acetic acid
• 5-(4-(benzoxazol-2-yl-5-acetic acid)phenylazo)-2-hydroxybenzoic acid
• Acetates
• Anti-Inflammatory Agents, Non-Steroidal
• Benzoxazoles
• Prodrugs
• Salicylates
• Mesalamine
• Trinitrobenzenesulfonic Acid

Topics

• Acetates (chemical synthesis, chemistry, pharmacology)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (chemical synthesis, chemistry, pharmacology)
• Benzoxazoles (chemical synthesis, chemistry, pharmacology)
• Colitis, Ulcerative (drug therapy, pathology)
• Disease Models, Animal
• Female
• Magnetic Resonance Spectroscopy
• Mass Spectrometry
• Mesalamine (pharmacology)
• Prodrugs
• Rats
• Rats, Wistar
• Salicylates (chemical synthesis, chemistry, pharmacology)
• Spectrophotometry, Infrared
• Trinitrobenzenesulfonic Acid (toxicity)