Abstract | SCOPE: METHODS AND RESULTS: Sera from 42 Spanish lettuce-allergic patients were obtained from patients recruited at the outpatient clinic. IgE- binding proteins were detected by SDS-PAGE and immunoblotting. Molecular characterization of IgE-binding bands was performed by MS. Thaumatin was purified using the Agilent 3100 OFFGEL system. The IgE-binding bands recognized in the sera of more than 50% of patients were identified as lipid transfer protein (9 kDa), a thaumatin-like protein (26 kDa), and an aspartyl protease (35 and 45 kDa). ELISA inhibition studies were performed to confirm the IgE reactivity of the purified allergen. CONCLUSION: Two new major lettuce allergens-a thaumatin-like protein and an aspartyl protease-have been identified and characterized. These allergens may be used to improve both diagnosis and treatment of lettuce-allergic patients.
|
Authors | Esther Muñoz-García, Olga Luengo-Sánchez, Elisa Haroun-Díaz, Aroa Sanz Maroto, Arancha Palacín, Araceli Díaz-Perales, Manuel de las Heras Gozalo, Moisés Labrador-Horrillo, Fernando Vivanco, Javier Cuesta-Herranz, Carlos Pastor-Vargas |
Journal | Molecular nutrition & food research
(Mol Nutr Food Res)
Vol. 57
Issue 12
Pg. 2245-52
(Dec 2013)
ISSN: 1613-4133 [Electronic] Germany |
PMID | 23983075
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Allergens
- Plant Proteins
- Immunoglobulin E
- thaumatin protein, plant
- Aspartic Acid Proteases
|
Topics |
- Adult
- Allergens
(immunology, isolation & purification)
- Amino Acid Sequence
- Aspartic Acid Proteases
(immunology, isolation & purification)
- Cross Reactions
(immunology)
- Electrophoresis, Polyacrylamide Gel
- Female
- Food Hypersensitivity
(immunology)
- Humans
- Immunoblotting
(methods)
- Immunoglobulin E
(metabolism)
- Lettuce
(immunology)
- Male
- Middle Aged
- Molecular Sequence Data
- Molecular Weight
- Plant Proteins
(immunology, isolation & purification)
- Prunus
(immunology)
- Young Adult
|