DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked is an immunogenic target of cancer stem cells.

Accumulating evidence suggests that most solid malignancies consist of heterogeneous tumor cells and that a relatively small subpopulation, which shares biological features with stem cells, survives through potentially lethal stresses such as chemotherapy and radiation treatment. Since the survival of this subpopulation of cancer stem cells (CSC) plays a critical role in recurrence, it must be eradicated in order to cure cancer. We previously reported that vaccination with CD133(+) murine melanoma cells exhibiting biological CSC features induced CSC-specific effector T cells. These were capable of eradicating CD133(+) tumor cells in vivo, thereby curing the parental tumor. In the current study, we indicated that DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked (DDX3X) is an immunogenic protein preferentially expressed in CD133(+) tumor cells. Vaccination with DDX3X primed specific T cells, resulting in protective and therapeutic antitumor immunity. The DDX3X-primed CD4(+) T cells produced CD133(+) tumor-specific IFN╬│ and IL-17 and mediated potent antitumor therapeutic efficacy. DDX3X is expressed in various human cancer cells, including lung, colon, and breast cancer cells. These results suggest that anti-DDX3X immunotherapy is a promising treatment option in efforts to eradicate CSC in the clinical setting.
AuthorsJun Koshio, Hiroshi Kagamu, Koichiro Nozaki, Yu Saida, Tomohiro Tanaka, Satoshi Shoji, Natsue Igarashi, Satoru Miura, Masaaki Okajima, Satoshi Watanabe, Hirohisa Yoshizawa, Ichiei Narita
JournalCancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 62 Issue 10 Pg. 1619-28 (Oct 2013) ISSN: 1432-0851 [Electronic] Germany
PMID23974721 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • AC133 antigen
  • Antigens, CD
  • Glycoproteins
  • Peptides
  • DDX3X protein, human
  • DEAD-box RNA Helicases
  • Animals
  • Antigens, CD (immunology)
  • DEAD-box RNA Helicases (immunology, metabolism)
  • Female
  • Glycoproteins (immunology)
  • Humans
  • Immunotherapy
  • Immunotherapy, Adoptive (methods)
  • Melanoma, Experimental (immunology, pathology, therapy)
  • Mice
  • Mice, Inbred C57BL
  • Neoplasms (immunology, metabolism, pathology, therapy)
  • Neoplastic Stem Cells (immunology, metabolism, pathology)
  • Peptides (immunology)

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