Abstract |
Cocaine- and amphetamine-regulated transcript peptides (CARTp) suppress nutritional intake after administration into the fourth intracerebral ventricle. Recent in vitro studies have shown that PACAP 6-38, a pituitary adenylate cyclase-activating polypeptide ( PACAP) fragment, could act as a competitive antagonist against CARTp 55-102 on a common CARTp-sensitive receptor structure. Here, we show for the first time in vivo that the reduction in solid food intake induced by exogenous CARTp 55-102 (0.3 nmol: 1.5 µg) administered fourth i.c.v. is blocked by pretreatment with PACAP 6-38 (3 nmol). The PACAP 6-38 fragment had no effect by itself either when given into the fourth ventricle or subcutaneously. Although effective to block the CARTp-effect on feeding and short-term body weight, PACAP 6-38 failed to attenuate CARTp-associated gross motor behavioral changes suggesting at least two CARTp-sensitive receptor subtypes. In conclusion, PACAP 6-38 acts as a functional CARTp antagonist in vivo and blocks its effects on feeding and short term weight gain.
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Authors | Jonathan R Burgos, Britt-Marie Iresjö, Ulrika Smedh |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 8
Pg. e72347
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23967296
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nerve Tissue Proteins
- Peptide Fragments
- Pituitary Adenylate Cyclase-Activating Polypeptide
- cocaine- and amphetamine-regulated transcript protein
- pituitary adenylate-cyclase-activating-peptide (6-38)
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Topics |
- Analysis of Variance
- Animals
- Feeding Behavior
(drug effects)
- Feeding and Eating Disorders
(drug therapy, etiology)
- Male
- Nerve Tissue Proteins
(antagonists & inhibitors)
- Peptide Fragments
(administration & dosage, pharmacology)
- Pituitary Adenylate Cyclase-Activating Polypeptide
(administration & dosage, pharmacology)
- Rats
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