Abstract | AIM: METHODS:
Tumor blocks were homogenized in a sterile grinder in saline. The homogenate was injected into the right armpit of each mouse. After vaccination, the mice were randomly assigned into four groups: a control group, a high dose HSV( GM-CSF) group [1 × 10⁷ plaque forming units (pfu)/ tumor], a medium dose HSV( GM-CSF) group (5 × 10⁶ pfu/ tumor) and a low dose HSV( GM-CSF) group (5 × 10⁵ pfu/ tumor). After initiation of drug administration, body weights and tumor diameters were measured every 3 d. Fifteen days later, after decapitation of the animal by cervical dislocation, each tumor was isolated, weighed and stored in 10% formaldehyde solution. The drug effectiveness was evaluated according to the weight, volume and relative volume change of each tumor. Furthermore, GM-CSF protein levels in serum were assayed by enzyme-linked immunosorbent assays at 1, 2, 3 and 4 d after injection of HSV( GM-CSF). RESULTS: Injection of the recombinant mouse HSV encoding GM-CSF resulted in a significant reduction in tumor growth compared to the control group, and dose-dependent effects were observed: the relative tumor proliferation rates of the low dose, medium dose and high dose groups on 15 d after injection were 45.5%, 55.2% and 65.5%, respectively. The inhibition rates of the tumor weights of the low, middle, and high dose groups were 41.4%, 46.7% and 50.5%, respectively. Furthermore, the production of GM-CSF was significantly increased in the mice infected with HSV( GM-CSF). The increase in the GM-CSF level was more pronounced in the high dose group compared to the other two dose groups. CONCLUSION: Our study provides the first evidence that HSV( GM-CSF) could inhibit the growth of pancreatic cancer. The enhanced GM-CSF expression might be responsible for the phenomenon.
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Authors | Hao Liu, Shou-Jun Yuan, Ying-Tai Chen, Yi-Bin Xie, Liang Cui, Wei-Zhi Yang, De-Xuan Yang, Yan-Tao Tian |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 19
Issue 31
Pg. 5138-43
(Aug 21 2013)
ISSN: 2219-2840 [Electronic] United States |
PMID | 23964149
(Publication Type: Journal Article)
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Chemical References |
- Granulocyte-Macrophage Colony-Stimulating Factor
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Topics |
- Animals
- Cell Line, Tumor
- Female
- Genetic Therapy
- Granulocyte-Macrophage Colony-Stimulating Factor
(genetics, metabolism)
- Mice
- Mice, Inbred C57BL
- Oncolytic Virotherapy
- Pancreatic Neoplasms
(genetics, metabolism, pathology, therapy, virology)
- Simplexvirus
(genetics, metabolism)
- Time Factors
- Tumor Burden
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