Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens.

Abstract

The human tumor-associated antigen CO-029 is a monoclonal antibody-defined cell surface glycoprotein of 27-34 kDa. By using the high-efficiency COS cell expression system, a full-length cDNA clone for CO-029 was isolated. When transiently expressed in COS cells, the cDNA clone directed the synthesis of an antigen reactive to monoclonal antibody CO-029 in mixed hemadsorption and immunoblot assays. Sequence analysis revealed that CO-029 belongs to a family of cell surface antigens that includes the melanoma-associated antigen ME491, the leukocyte cell surface antigen CD37, and the Sm23 antigen of the parasitic helminth Schistosoma mansoni. CO-029 and ME491 antigen expression and the effect of their corresponding monoclonal antibodies on cell growth were compared in human tumor cell lines of various histologic origins.

• Authors: S Szala, Y Kasai, Z Steplewski, U Rodeck, H Koprowski, A J Linnenbach
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 87 Issue 17 Pg. 6833-7 (Sep 1990) ISSN: 0027-8424 [Print] United States
• PMID: 2395876 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• DNA, Neoplasm
• Membrane Glycoproteins
• TSPAN8 protein, human
• Tetraspanins

Topics

• Amino Acid Sequence
• Animals
• Antigens, Neoplasm (genetics)
• Base Sequence
• Cell Line
• Cell Membrane (ultrastructure)
• Cloning, Molecular
• DNA, Neoplasm (genetics)
• Humans
• Membrane Glycoproteins (genetics)
• Models, Structural
• Molecular Sequence Data
• Protein Conformation
• Sequence Homology, Nucleic Acid
• Tetraspanins
• Transfection