Interleukin 2 receptor beta chain expressed in an oligodendroglioma line binds interleukin 2 and delivers growth signal.

Abstract

Interleukin 2 (IL-2) is a potent growth factor for T lymphocytes, playing a crucial role in the immune response. In view of the considerable evidence that the immunoregulatory cytokines (or lymphokines) also play a role in the growth and differentiation of cells in the central nervous system (CNS), we examined the operation of the IL-2 system in a cell line of CNS origin by expressing a cDNA encoding the beta chain of the human IL-2 receptor (IL-2R beta, a 75-kDa protein). When the cDNA was expressed in a human oligodendroglioma cell line, ONS-21, the IL-2R beta bound IL-2 with an affinity similar to that in lymphoid cells (Kd, approximately 2 nM). Furthermore, cell proliferation ([3H]thymidine incorporation) was stimulated by IL-2. These results demonstrate that the same cytokine receptor is functional in cells of the immune system and CNS and point to a molecular mechanism that is similar for growth-signal transduction between lymphoid and neural cells but that may be different in other cells, such as fibroblasts.

• Authors: Y Okamoto, S Minamoto, K Shimizu, H Mogami, T Taniguchi
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 87 Issue 17 Pg. 6584-8 (Sep 1990) ISSN: 0027-8424 [Print] United States
• PMID: 2395860 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Interleukin-2
• Macromolecular Substances
• Receptors, Interleukin-2

Topics

• Antibodies
• Cell Division (drug effects)
• Cell Line
• Clone Cells
• DNA Replication (drug effects)
• Humans
• Interleukin-2 (immunology, metabolism, pharmacology)
• Kinetics
• Macromolecular Substances
• Oligodendroglioma
• Receptors, Interleukin-2 (genetics, metabolism, physiology)
• Transfection