Identification of genuine primary pulmonary NK cell lymphoma via clinicopathologic observation and clonality assay.

Abstract

Extranodal natural killer (NK)/T-cell lymphoma, nasal type, is an uncommon lymphoma associated with the Epstein-Barr virus (EBV). It most commonly involves the nasal cavity and upper respiratory tract. Primary pulmonary NK/T cell lymphoma is extremely rare. If a patient with a NK or T-cell tumor has an unusual reaction to treatment or an unusual prognosis, it is wise to differentiate NK from T-cell tumors. The clinicopathologic characteristics, immunophenotype, EBV in situ hybridization, and T cell receptor (TCR) gene rearrangement of primary pulmonary NK cell lymphoma from a 73-year-old Chinese woman were investigated and the clonal status was determined using female X-chromosomal inactivation mosaicism and polymorphisms at the phosphoglycerate kinase (PGK) gene. The lesion showed the typical histopathologic characteristics and immunohistochemical features of NK/T cell lymphoma. However, the sample was negative for TCR gene rearrangement. A clonality assay demonstrated that the lesion was monoclonal. It is concluded that this is the first recorded case of genuine primary pulmonary NK cell lymphoma. The purpose of the present work is to recommend that pathologists carefully investigate the whole lesion to reduce the likelihood that primary pulmonary NK cell lymphoma will be misdiagnosed as an infectious lesion. In addition, TCR gene rearrangement and clonal analysis, which is based on female X-chromosomal inactivation mosaicism and polymorphisms at PGK and androgen receptor (AR) loci, were found to play important roles in differentiating NK cell lymphoma from T cell lymphoma.

VIRTUAL SLIDES:

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• Authors: Li Gong, Long-Xiao Wei, Gao-Sheng Huang, Wen-Dong Zhang, Lu Wang, Shao-Jun Zhu, Xiu-Juan Han, Li Yao, Miao Lan, Yan-Hong Li, Wei Zhang
• Journal: Diagnostic pathology (Diagn Pathol) Vol. 8 Pg. 140 (Aug 19 2013) ISSN: 1746-1596 [Electronic] England
• PMID: 23958352 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AR protein, human
• Receptors, Androgen
• Phosphoglycerate Kinase

Topics

• Aged
• Chromosome Disorders
• Fatal Outcome
• Female
• Gene Rearrangement, T-Lymphocyte
• Genetic Predisposition to Disease
• Genetic Testing (methods)
• Herpesvirus 4, Human (genetics)
• Humans
• Immunophenotyping
• In Situ Hybridization
• Lung Neoplasms (genetics, immunology, pathology, virology)
• Lymphoma, Extranodal NK-T-Cell (genetics, immunology, pathology, virology)
• Mosaicism
• Phenotype
• Phosphoglycerate Kinase (genetics)
• Polymerase Chain Reaction
• Polymorphism, Single Nucleotide
• Predictive Value of Tests
• Receptors, Androgen (genetics)
• Tomography, X-Ray Computed
• Treatment Refusal
• X Chromosome Inactivation