Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype.

Abstract

During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11b(high), but not CD11b(low) macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11b(high) to a CD11b(low) phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation.

• Authors: Ran Rostoker, Hiba Yaseen, Sagie Schif-Zuck, Rachel G Lichtenstein, Gabriel A Rabinovich, Amiram Ariel
• Journal: Prostaglandins & other lipid mediators (Prostaglandins Other Lipid Mediat) Vol. 107 Pg. 85-94 (Dec 2013) ISSN: 1098-8823 [Print] United States
• PMID: 23954858 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• CD11 Antigens
• Cytokines
• Galectin 1
• Lipopolysaccharides
• Arachidonate 12-Lipoxygenase
• Arachidonate 15-Lipoxygenase

Topics

• Animals
• Apoptosis
• Arachidonate 12-Lipoxygenase (genetics, metabolism)
• Arachidonate 15-Lipoxygenase (genetics, metabolism)
• CD11 Antigens (metabolism)
• Cells, Cultured
• Cytokines (metabolism)
• Enzyme Induction
• Galectin 1 (physiology)
• Inflammation (enzymology)
• Lipopolysaccharides (pharmacology)
• Macrophages, Peritoneal (enzymology, immunology, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Phenotype