Luteolin 8-C-β-fucopyranoside inhibits invasion and suppresses TPA-induced MMP-9 and IL-8 via ERK/AP-1 and ERK/NF-κB signaling in MCF-7 breast cancer cells.

Abstract

Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast cancer cells. We investigated whether LU8C-FP would inhibit MMP-9 activation and IL-8 expression in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 breast cancer cells. LU8C-FP suppressed TPA-induced MMP-9 and IL-8 secretion and mRNA expression via inhibition of the MAPK signaling pathway and down-regulation of nuclear AP-1 and NF-κB. TPA-induced phosphorylation of ERK 1/2 was suppressed by LU8C-FP, whereas JNK and p38 MAPK phosphorylation were unaffected. In addition, LU8C-FP blocked the ERK 1/2 pathways following expression of MMP-9 and IL-8. These results suggest LU8C-FP may function to suppress invasion of breast cancer cells through the ERK/AP-1 and ERK/NF-κB signaling cascades.

• Authors: Su-Ho Park, Jung-Hee Kim, Dong-Hun Lee, Jeong-Woo Kang, Hyuk-Hwan Song, Sei-Ryang Oh, Do-Young Yoon
• Journal: Biochimie (Biochimie) Vol. 95 Issue 11 Pg. 2082-90 (Nov 2013) ISSN: 1638-6183 [Electronic] France
• PMID: 23933110 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright © 2013. Published by Elsevier Masson SAS. All rights reserved.

Chemical References

• Interleukin-8
• NF-kappa B
• Transcription Factor AP-1
• Matrix Metalloproteinase 9
• Luteolin
• Tetradecanoylphorbol Acetate

Topics

• Breast Neoplasms (drug therapy, genetics, pathology)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Interleukin-8 (biosynthesis, genetics)
• Luteolin (pharmacology)
• MAP Kinase Signaling System (drug effects)
• MCF-7 Cells
• Matrix Metalloproteinase 9 (biosynthesis, genetics)
• NF-kappa B (metabolism)
• Signal Transduction (drug effects)
• Tetradecanoylphorbol Acetate (pharmacology)
• Transcription Factor AP-1 (metabolism)