Abstract | HYPOTHESIS: Using an appropriate dose of an aminoglycoside antibiotic cotreated with a loop diuretic a guinea pig model of high-frequency loss can be obtained mimicking cochlear implant candidates with low-frequency residual hearing. We examined the stability of this model over time. BACKGROUND: METHODS: Guinea pigs received an electrode for chronic recording of compound action potentials to tones to assess thresholds. They were treated with a coadministration of kanamycin (200 mg/kg) and furosemide (100 mg/kg), after which, the animals were sacrificed for histologic analysis at 2, 4, or 7 weeks. RESULTS: After 2 to 7 weeks threshold shifts were greater than 50 dB for 8 to 16 kHz in 15 of 17 animals, whereas threshold shifts at 2 kHz or lower were less than 50 dB in 13 animals. Major threshold shifts occurred the first 2 to 4 days; subsequently, some spontaneous recovery occurred and, after 2-3 weeks thresholds, remained stable. Inner hair cell loss still progressed between 2 and 4 weeks in the most basal cochlear region; thereafter, hair cell loss was stable. CONCLUSION: An appropriate animal model for selective severe high-frequency hearing loss was obtained, which is stable at 4 weeks after ototoxic treatment.
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Authors | Sarah Havenith, Sjaak F L Klis, Huib Versnel, Wilko Grolman |
Journal | Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology
(Otol Neurotol)
Vol. 34
Issue 8
Pg. 1510-8
(Oct 2013)
ISSN: 1537-4505 [Electronic] United States |
PMID | 23928512
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Action Potentials
(physiology)
- Animals
- Cochlea
(pathology, physiopathology)
- Disease Models, Animal
- Furosemide
- Guinea Pigs
- Hair Cells, Auditory
(pathology, physiology)
- Hearing
(physiology)
- Hearing Loss, High-Frequency
(chemically induced, pathology, physiopathology)
- Kanamycin
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