Abstract | BACKGROUND: STUDY DESIGN AND METHODS:
Adenine nucleotide contents, RBC morphology, and plasma free hemoglobin (PF-Hb) level of RBCs treated with NS or MAP solution were compared under three conditions: (1) 4-hour preservation of fresh blood from healthy volunteers, (2) collection from the shed blood of patients, and 3) incubation of the collected shed blood with plasma. RESULTS:
Adenine nucleotide level and RBC elongation index were greater and PF-Hb level was lower in MAP groups than NS groups (p < 0.05) after preservation and incubation. In NS, RBCs lost their deformability and became stomatocytes, and even RBC "ghosts" 48 hours after incubation, while they remained normal in MAP solution. CONCLUSION: The MAP solution helps preserve RBC morphology and function, and reduces hemolysis, possibly due to improved energy production. Therefore, MAP should replace NS during IBS.
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Authors | Ping Yang, Jing Zhou, Yi Kang, Lina Gong, Jie Zhang, Jie Yu, Xiaorong Yin, Chongwei Zhang, Guangyue Liu, Jin Liu, Lei Du |
Journal | Transfusion
(Transfusion)
Vol. 54
Issue 4
Pg. 1146-52
(Apr 2014)
ISSN: 1537-2995 [Electronic] United States |
PMID | 23927791
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 American Association of Blood Banks. |
Chemical References |
- Mannitol Phosphates
- Adenine
|
Topics |
- Adenine
(chemistry, pharmacology, therapeutic use)
- Blood Preservation
(methods)
- Cell Shape
(drug effects)
- Cells, Cultured
- Drug Evaluation, Preclinical
- Erythrocyte Deformability
(drug effects)
- Erythrocytes
(cytology, drug effects, physiology)
- Humans
- Mannitol Phosphates
(chemistry, pharmacology, therapeutic use)
- Operative Blood Salvage
(methods)
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