Abstract |
Antibody avidity for antigens following disease or vaccination increases with affinity maturation and somatic hypermutation. In this study, we followed children and adults in Bangladesh for 1 year following oral cholera vaccination and measured the avidity of antibodies to the T cell-dependent antigen cholera toxin B subunit (CTB) and the T cell-independent antigen lipopolysaccharide (LPS) in comparison with responses in other immunological measurements. Children produced CTB-specific IgG and IgA antibodies of high avidity following vaccination, which persisted for several months; the magnitudes of responses were comparable to those seen in adult vaccinees. The avidity of LPS-specific IgG and IgA antibodies in vaccinees increased significantly shortly after the second dose of vaccine but waned rapidly to baseline levels thereafter. CTB-specific memory B cells were present for only a short time following vaccination, and we did not find significant memory B cell responses to LPS in any age group. For older children, there was a significant correlation between CTB-specific memory T cell responses after the second dose of vaccine and CTB-specific IgG antibody avidity indices over the subsequent year. These findings suggest that vaccination induces a longer-lasting increase in the avidity of antibodies to a T cell-dependent antigen than is measured by a memory B cell response to that antigen and that early memory T cell responses correlate well with the subsequent development of higher-avidity antibodies.
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Authors | Mohammad Murshid Alam, Daniel T Leung, Marjahan Akhtar, Mohammad Nazim, Sarmin Akter, Taher Uddin, Farhana Khanam, Deena Al Mahbuba, Shaikh Meshbahuddin Ahmad, Taufiqur Rahman Bhuiyan, Stephen B Calderwood, Edward T Ryan, Firdausi Qadri |
Journal | Clinical and vaccine immunology : CVI
(Clin Vaccine Immunol)
Vol. 20
Issue 10
Pg. 1541-8
(Oct 2013)
ISSN: 1556-679X [Electronic] United States |
PMID | 23925888
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Bacterial
- Cholera Vaccines
- Immunoglobulin A
- Immunoglobulin G
- Lipopolysaccharides
- Vaccines, Inactivated
- Cholera Toxin
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Topics |
- Administration, Oral
- Adolescent
- Adult
- Antibodies, Bacterial
(blood)
- Antibody Affinity
- B-Lymphocytes
(immunology)
- Bangladesh
- Child
- Child, Preschool
- Cholera Toxin
(immunology)
- Cholera Vaccines
(administration & dosage, immunology)
- Female
- Humans
- Immunoglobulin A
(blood)
- Immunoglobulin G
(blood)
- Immunologic Memory
- Lipopolysaccharides
(immunology)
- Male
- Middle Aged
- Time Factors
- Vaccines, Inactivated
(administration & dosage, immunology)
- Young Adult
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