Ovarian cyst fluids are a cache of tumor biomarkers that include calgranulin A and calgranulin B isoforms.

Abstract

SELDI-TOF MS analysis of cyst fluids identified 95 peaks that discriminate malignant, borderline, and benign ovarian tumors. Three prominent peaks, which correspond to calgranulin A (m/z 10847) and two isoforms of calgranulin B (m/z 12717 and 13294), have higher concentrations in borderline and malignant cyst fluids. Together, calgranulin A and B distinguish borderline and malignant tumors from benign tumors with 28.6% and 63.6% sensitivity for early stage disease, respectively, at 95% specificity and with 74.8% accuracy. Ovarian cyst fluids are useful for discovering discriminatory biomarkers, such as calgranulin, which may have utility for detecting, diagnosing, and biochemically classifying ovarian tumors.

• Authors: Hollie S Skaggs, Brook A Saunders, Rachel W Miller, Scott T Goodrich, Monica S King, Kimberly D Kimbler, Adam J Branscum, Eric T Fung, Paul D DePriest, John R van Nagell, Frederick R Ueland, Andre T Baron
• Journal: Cancer investigation (Cancer Invest) Vol. 31 Issue 7 Pg. 433-53 (Aug 2013) ISSN: 1532-4192 [Electronic] England
• PMID: 23915068 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Calgranulin A
• Calgranulin B
• Protein Isoforms

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (analysis)
• Blotting, Western
• Calgranulin A (analysis, biosynthesis)
• Calgranulin B (analysis, biosynthesis)
• Cyst Fluid (chemistry)
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Middle Aged
• Ovarian Cysts (chemistry)
• Ovarian Neoplasms (diagnosis, metabolism)
• Protein Isoforms (analysis, biosynthesis)
• Sensitivity and Specificity
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization