The MFS-type efflux pump Flr1 induced by Yap1 promotes canthin-6-one resistance in yeast.

Abstract

Screening for suppressors of canthin-6-one toxicity in yeast identified Yap1, a transcription factor involved in cell response to a broad range of injuries. Although canthin-6-one did not promote a significant oxidative stress, overexpression of YAP1 gene clearly increased resistance to this drug. We demonstrated that Yap1-mediated resistance involves the plasma membrane major-facilitator-superfamily efflux pump Flr1 but not the vacuolar ATP-binding-cassette transporter Ycf1. FLR1 overexpression was sufficient to reduce sensitivity to the drug, but strictly dependent on a functional YAP1 gene.

• Authors: Camille Dejos, Matthieu Régnacq, Marianne Bernard, Pierre Voisin, Thierry Bergès
• Journal: FEBS letters (FEBS Lett) Vol. 587 Issue 18 Pg. 3045-51 (Sep 17 2013) ISSN: 1873-3468 [Electronic] England
• PMID: 23912082 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Chemical References

• ATP-Binding Cassette Transporters
• Carbolines
• FLR1 protein, S cerevisiae
• Fungicides, Industrial
• Indole Alkaloids
• Organic Anion Transporters
• Saccharomyces cerevisiae Proteins
• Transcription Factors
• YAP1 protein, S cerevisiae
• YCF1 protein, S cerevisiae
• canthin-6-one

Topics

• ATP-Binding Cassette Transporters (genetics, metabolism)
• Carbolines (pharmacology)
• Cell Membrane (drug effects)
• Drug Resistance, Fungal (drug effects, genetics)
• Fungicides, Industrial (pharmacology)
• Gene Expression Regulation, Fungal (drug effects)
• Indole Alkaloids (pharmacology)
• Organic Anion Transporters (genetics, metabolism)
• Saccharomyces cerevisiae (drug effects, genetics, metabolism)
• Saccharomyces cerevisiae Proteins (genetics, metabolism)
• Signal Transduction
• Transcription Factors (genetics, metabolism)