Role of PON2 in innate immune response in an acute infection model.

Abstract

N-(3-oxododecanoyl)-l-homoserine lactone (3OC(12)-HSL) is a quorum-sensing molecule produced by gram-negative microbial pathogens such as Pseudomonas aeruginosa (PAO1). 3OC(12)-HSL is involved in the regulation of bacterial virulence factors and also alters the function of the host immune cells. Others and we have previously shown that paraoxonase 2 (PON2), a member of the paraoxonase gene family expressed in immune cells, hydrolyzes 3OC(12)-HSL. In this study, we examined i) whether macrophage PON2 participates in 3OC(12)-HSL hydrolysis, ii) the effect of PON2 deficiency in acute PAO1 infection in mice and iii) the effect of 3OC(12)-HSL on PON2 deficient (PON2-def) macrophages. When compared to wild type macrophages, both intact cells and membrane-enriched protein lysates obtained from PON2-def macrophages show a marked impairment in their ability to hydrolyze 3OC(12)-HSL. PON2 expression (message and protein) is not altered in response to 3OC(12)-HSL in macrophages. 3OC(12)-HSL treated PON2-def macrophages showed i) an increase in ER stress and oxidative stress, ii) defective phosphatidylinositol 3-kinase (PI3 kinase)/AKT activation, and iii) reduced phagocytosis function. Moreover, the nitration to phosphorylation ratio of Tyr458 in p85 protein, the regulatory subunit of PI3-kinase that has been correlated with the phagocytosis function of macrophages, was increased in PON2-def macrophages. Antioxidant treatment reversed the effects of PON2 deficiency in macrophage phagocytosis function. Furthermore, following administration of 1.6 × 10(7) CFU of PAO1, bacterial clearance was significantly reduced in the lungs (5.7 fold), liver (2.5 fold), and spleen (14.8 fold) of PON2-def mice when compared to wild type mice. Our results suggest that PON2 plays an important role in innate immune defense against PAO1 infection.

• Authors: Asokan Devarajan, Noam Bourquard, Victor R Grijalva, Feng Gao, Ekambaram Ganapathy, Jitendra Verma, Srinivasa T Reddy
• Journal: Molecular genetics and metabolism (Mol Genet Metab) Vol. 110 Issue 3 Pg. 362-70 (Nov 2013) ISSN: 1096-7206 [Electronic] United States
• PMID: 23911207 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2013.

Chemical References

• Antioxidants
• Inflammation Mediators
• N-(3-oxododecanoyl)homoserine lactone
• Reactive Oxygen Species
• Homoserine
• PON2 protein, mouse
• Aryldialkylphosphatase
• 4-Butyrolactone

Topics

• 4-Butyrolactone (analogs & derivatives, pharmacology)
• Animals
• Antioxidants (pharmacology)
• Aryldialkylphosphatase (genetics)
• Homoserine (analogs & derivatives, pharmacology)
• Immunity, Innate (genetics)
• Inflammation Mediators (metabolism)
• Macrophages (drug effects, immunology, microbiology)
• Male
• Mice
• Mice, Knockout
• Oxidative Stress
• Phagocytosis (drug effects, genetics, immunology)
• Pseudomonas Infections (genetics, immunology)
• Pseudomonas aeruginosa (immunology)
• Quorum Sensing (immunology)
• Reactive Oxygen Species