The efficacy of combination therapy using adeno-associated virus-mediated co-expression of apoptin and interleukin-24 on hepatocellular carcinoma.

Abstract

Multigene-based combination therapy is an effective practice in cancer gene therapy. Apoptin is a chicken anemia virus-derived, p53-independent, Bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in various human tumor cells. Interleukin-24 (IL-24) displays ubiquitous antitumor property and tumor-specific killing activity. Adeno-associated virus (AAV) is a promising gene delivery vehicle due to its advantage of low pathogenicity and long-term gene expression. In this study, we assessed the efficacy of combination therapy using AAV-mediated co-expression of apoptin and interleukin-24 on hepatocellular carcinoma in vitro and in vivo. Our results showed that AAV-mediated co-expression of IL-24 and apoptin significantly suppressed the growth and induced the apoptosis of HepG2 cells in vitro. Furthermore, AAV-mediated combined treatment of IL-24 and apoptin significantly suppressed tumor growth and induced apoptosis of tumor cells in xenograft nude mice. These data suggest that AAV vectors that co-express apoptin and IL-24 have great potential in cancer gene therapy.

• Authors: Lijie Yuan, Hengyu Zhao, Liqiu Zhang, Xinghan Liu
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 34 Issue 5 Pg. 3027-34 (Oct 2013) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 23907578 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apoptosis Regulatory Proteins
• Capsid Proteins
• Interleukins
• VP3 protein, Chicken anemia virus
• interleukin-24

Topics

• Animals
• Apoptosis
• Apoptosis Regulatory Proteins (metabolism)
• Capsid Proteins (biosynthesis, genetics)
• Carcinoma, Hepatocellular (metabolism, pathology, therapy)
• Dependovirus (genetics)
• Gene Expression
• Genetic Therapy
• Genetic Vectors
• Hep G2 Cells
• Humans
• Interleukins (biosynthesis, genetics)
• Liver Neoplasms (metabolism, pathology, therapy)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Transplantation
• Tumor Burden