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Intranuclear drug delivery and effective in vivo cancer therapy via estradiol-PEG-appended multiwalled carbon nanotubes.

Abstract
Cancer cell-selective, nuclear targeting is expected to enhance the therapeutic efficacy of a myriad of antineoplastic drugs, particularly those whose pharmacodynamic site of action is the nucleus. In this study, a steroid-macromolecular bioconjugate based on PEG-linked 17β-Estradiol (E2) was appended to intrinsically cell-penetrable multiwalled carbon nanotubes (MWCNTs) for intranuclear drug delivery and effective breast cancer treatment, both in vitro and in vivo. Taking Doxorubicin (DOX) as a model anticancer agent, we tried to elucidate how E2 appendage influences the cell internalization, intracellular trafficking, and antitumor efficacy of the supramolecularly complexed drug. We observed that the combination of DOX with E2-PEG-MWCNTs not only facilitated nuclear targeting through an estrogen receptor (ER)-mediated pathway but also deciphered to a synergistic anticancer response in vivo. The antitumor efficacy of DOX@E2-PEG-MWCNTs in chemically breast cancer-induced female rats was approximately 18, 17, 5, and 2 times higher compared to the groups exposed to saline, drug-deprived E2-PEG-MWCNTs, free DOX, and DOX@m-PEG-MWCNTs, respectively. While free DOX treatment induced severe cardiotoxicity in animals, animals treated with DOX@m-PEG-MWCNTs and DOX@E2-PEG-MWCNTs were devoid of any perceivable cardiotoxicity, hepatotoxicity, and nephrotoxicity. To the best of our knowledge, this is the first instance in which cancer cell-selective, intranuclear drug delivery, and, subsequently, effective in vivo breast cancer therapy has been achieved using estrogen-appended MWCNTs as the molecular transporter.
AuthorsManasmita Das, Raman Preet Singh, Satyajit R Datir, Sanyog Jain
JournalMolecular pharmaceutics (Mol Pharm) Vol. 10 Issue 9 Pg. 3404-16 (Sep 03 2013) ISSN: 1543-8392 [Electronic] United States
PMID23905512 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Nanotubes, Carbon
  • Polymers
  • Estradiol
  • Ritonavir
Topics
  • Calorimetry, Differential Scanning
  • Drug Delivery Systems (methods)
  • Estradiol (chemistry)
  • Molecular Structure
  • Nanotubes, Carbon (chemistry)
  • Polymers (chemistry)
  • Ritonavir (chemistry)
  • Spectrometry, Fluorescence

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