Clinical potential of a silk sericin-releasing bioactive wound dressing for the treatment of split-thickness skin graft donor sites.

Abstract

PURPOSE:

An ethyl alcohol-precipitated silk sericin/PVA scaffold that controlled the release of silk sericin was previously developed and applied for the treatment of full-thickness wounds in rats and demonstrated efficient healing. In this study, we aimed to further evaluate the clinical potential of this scaffold, hereafter called "silk sericin-releasing wound dressing", for the treatment of split-thickness skin graft donor sites by comparison with the clinically available wound dressing known as "Bactigras®".

METHODS:

In vitro characterization and in vivo evaluation for safety of the wound dressings were performed. A clinical trial of the wound dressings was conducted according to standard protocols.

RESULTS:

The sericin released from the wound dressing was not toxic to HaCat human keratinocytes. A peel test indicated that the silk sericin-releasing wound dressing was less adhesive than Bactigras®, potentially reducing trauma and the risk of repeated injury upon removal. There was no evidence of skin irritation upon treatment with either wound dressing. When tested in patients with split-thickness skin graft donor sites, the wounds treated with the silk sericin-releasing wound dressing exhibited complete healing at 12 ± 5.0 days, whereas those treated with Bactigras® were completely healed at 14 ± 5.2 days (p = 1.99 × 10(-4)). In addition, treatment with the silk sericin-releasing wound dressing significantly reduced pain compared with Bactigras® particularly during the first 4 postoperative days (p = 2.70 × 10(-5) on day 1).

CONCLUSION:

We introduce this novel silk sericin-releasing wound dressing as an alternative treatment for split-thickness skin graft donor sites.

• Authors: Tippawan Siritientong, Apichai Angspatt, Juthamas Ratanavaraporn, Pornanong Aramwit
• Journal: Pharmaceutical research (Pharm Res) Vol. 31 Issue 1 Pg. 104-16 (Jan 2014) ISSN: 1573-904X [Electronic] United States
• PMID: 23900888 (Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Adhesives
• Sericins
• Silk
• Oxygen

Topics

• Adhesives
• Adult
• Animals
• Bandages
• Cell Cycle (drug effects)
• Cell Survival (drug effects)
• Female
• Humans
• Keratinocytes (drug effects)
• Male
• Middle Aged
• Oxygen (metabolism)
• Prospective Studies
• Rats
• Rats, Wistar
• Sericins (pharmacology)
• Silk (pharmacology)
• Skin Transplantation (methods)
• Swine
• Wound Healing (drug effects)
• Young Adult