Abstract |
CD176 ( Thomsen-Friedenreich antigen) is a tumor-associated carbohydrate structure. CD176 is expressed at the surface of human leukemic cells but is almost absent in normal and benign adult human tissues. Therefore, CD176 could be a promising target for antitumor immunotherapy. In the present study, pre-immunization with asialoglycophorin A (containing the CD176 oligosaccharide chains) was able to significantly improve the survival time of mice carrying CD176+ leukemia as compared to the control mice without the immunization. Furthermore, the passive transfer of CD176 antiserum which reacted only with the tumor-associated CD176 in cancer cells, was able to effectively prolong the survival time of CD176+ leukemia mice. In particular, the CD176 antiserum treatment could inhibit the growth and spreading of CD176+ leukemic cells in bone marrow, spleen, liver, and lung as evidenced by histopathological examination. CD176 antiserum could induce the apoptosis of CD176+ leukemic cells in vivo in a manner as previously observed in vitro. The data provided strong evidence that both CD176 antigen-based active immunotherapy and CD176 antibody-based passive immunotherapy lead to a therapeutic response in CD176+ leukemia mice. Therefore, both CD176 vaccine and CD176 antibody drug may be beneficial for the treatment of CD176+ leukemia patients.
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Authors | Bin Yi, Zhe Zhang, Min Zhang, Reinhard Schwartz-Albiez, Yi Cao |
Journal | Oncology reports
(Oncol Rep)
Vol. 30
Issue 4
Pg. 1841-7
(Oct 2013)
ISSN: 1791-2431 [Electronic] Greece |
PMID | 23900643
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies
- Antigens, Tumor-Associated, Carbohydrate
- Asialoglycoproteins
- Cancer Vaccines
- Immune Sera
- asialoglycophorin AM
- Thomsen-Friedenreich antigen
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Topics |
- Animals
- Antibodies
(immunology)
- Antigens, Tumor-Associated, Carbohydrate
(immunology, metabolism)
- Apoptosis
(immunology)
- Asialoglycoproteins
(immunology)
- Bone Marrow
(immunology)
- Cancer Vaccines
(immunology)
- Cell Line, Tumor
- Female
- Humans
- Immune Sera
(administration & dosage)
- Immunization, Passive
(methods)
- Immunotherapy, Active
(methods)
- Leukemia
(therapy)
- Male
- Mice
- Mice, Inbred BALB C
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