An immunohistochemical panel to distinguish ovarian from uterine serous papillary carcinomas.

Abstract

Serous papillary carcinomas (SPCs) share a similar morphology regardless of whether they originate from the ovary or the uterus. Identification of the site of origin of the tumor can be a challenging and a diagnostic dilemma, particularly, in the setting of a pelvic mass or peritoneal carcinomatosis. Recognition of the site of origin influences the staging, management, and prognosis of these malignancies. The purpose of this study is to identify a panel of markers to distinguish the ovarian serous papillary carcinomas (OSPC) from the uterine serous papillary carcinomas (USPC). Formalin-fixed, paraffin-embedded archival tissue from 46 cases of SPCs (33 uterine and 13 ovarian) were stained using antibodies for estrogen receptor (ER), WT1, insulin-like growth factor-II mRNA-binding protein 3 (IMP3), p53, and p16. The OSPC expressed ER (92%), WT1 (100%), IMP3 (92%), p53 (92%), and p16 (92%). The USPCs expressed ER (30%), WT1 (64%), IMP3 (85%), p53 (64%), and p16 (76%). Only ER expression was significantly higher in OSPC compared with USPCs (P<0.001). The combined ER(+)WT1(+) phenotype was present in 92% of the OSPC, whereas only 18% of the USPCs had the same phenotype (P<0.001). Furthermore, 71% of the OSPCs expressed ER(+), p53(+), WT1(+), IMP3(+), and p16(+) immunophenotype, whereas in USPCs, the tumor cells showed immunophenotypic diversity, with only 6% of the USPCs expressing reactivity to all the 5 markers (P<0.001). This study suggests that ER alone or in combination with a limited panel of markers may be used to identify the site of origin of SPCs.

• Authors: Yaxia Zhang, Monica T Garcia-Buitrago, Tulay Koru-Sengul, Samer Schuman, Parvin Ganjei-Azar
• Journal: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (Int J Gynecol Pathol) Vol. 32 Issue 5 Pg. 476-81 (Sep 2013) ISSN: 1538-7151 [Electronic] United States
• PMID: 23896709 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor

Topics

• Biomarkers, Tumor (metabolism)
• Carcinoma, Papillary (diagnosis, metabolism, pathology)
• Cystadenocarcinoma, Serous (diagnosis, metabolism, pathology)
• Diagnosis, Differential
• Female
• Humans
• Immunohistochemistry
• Ovarian Neoplasms (diagnosis, metabolism, pathology)
• Ovary (metabolism, pathology)
• Uterine Neoplasms (diagnosis, metabolism, pathology)
• Uterus (metabolism, pathology)