Abstract | OBJECTIVES: MATERIALS AND METHODS: Saliva samples were collected from five study groups (25 subjects/group): newly diagnosed OSCC, OSCC-in-remission, disease-active OLP, disease-inactive OLP, and normal controls. The salivary mRNA levels were determined by a pre-amplification RT-qPCR approach with nested gene-specific primers. Mean fold changes between each pair of study groups were analyzed by the Mann-Whitney U test. RESULTS: Salivary levels of OAZ1, S100P, and DUSP1 mRNAs were significantly higher in newly diagnosed OSCC patients, compared to: (1) normal controls (p = 0.003; p = 0.003; and p < 0.001, respectively); (2) OSCC-in-remission (p < 0.001; p = 0.001; and p < 0.001, respectively); (3) disease-active OLP (p < 0.001; p = 0.016; and p < 0.001, respectively); and (4) disease-inactive OLP (p = 0.043; p < 0.001; and p < 0.001, respectively). No significant differences were found in the levels of salivary IL-8, IL-1β, H3F3A, and SAT1 mRNAs between newly diagnosed OSCC patients and the normal controls (p = 0.093, 0.327, 0.764, and 0.560, respectively). CONCLUSION: Salivary OAZ1, S100P, and DUSP1 mRNAs are candidate biomarkers for detecting OSCC development in OSCC patients in remission and in OLP patients. CLINICAL RELEVANCE: The results of this study serve as the basis for a further large-scale study which may lead to a non-invasive screening method for early detection of OSCC.
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Authors | Yi-Shing Lisa Cheng, Lee Jordan, Terry Rees, Huey-Shys Chen, Lance Oxford, Ole Brinkmann, David Wong |
Journal | Clinical oral investigations
(Clin Oral Investig)
Vol. 18
Issue 3
Pg. 985-93
(Apr 2014)
ISSN: 1436-3771 [Electronic] Germany |
PMID | 23892499
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Biomarkers, Tumor
- RNA, Messenger
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Topics |
- Biomarkers, Tumor
(metabolism)
- Humans
- Lichen Planus, Oral
(metabolism)
- Mouth Neoplasms
(metabolism)
- RNA, Messenger
(metabolism)
- Remission Induction
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