Application of (99m)Technetium-HYNIC(tricine/TPPTS)-Aca-Bombesin(7-14) SPECT/CT in prostate cancer patients: a first-in-man study.

Abstract

RATIONALE:

The peptide bombesin (BBN) and its derivatives exhibit high binding affinity for the gastrin-releasing peptide receptor (GRPR), which is highly expressed in prostate cancer. We used the BBN-based radiopharmaceutical (99m)Technetium-HYNIC(tricine/TPPTS)-Aca-Bombesin(7-14) ((99m)Tc-HABBN) to perform a first-in-man clinical pilot study to evaluate the feasibility of (99m)Tc-HABBN SPECT/CT for detection of prostate cancer in patients.

METHODS:

Eight patients with biopsy-proven prostate cancer who were scheduled for either radical prostatectomy or external beam radiotherapy underwent (99m)Tc-HABBN scintigraphy and SPECT/CT prior to treatment. Serial blood samples were taken to assess blood radioactivity and to determine in vivo metabolic stability. Clinical parameters were measured and reported side effects, if present, were recorded. Prostate cancer specimens of all patients were immunohistochemically stained for GRPR.

RESULTS:

(99m)Tc-HABBN was synthesized with high radiochemical yield, purity and specific activity. There were no significant changes in clinical parameters, and there were no adverse or subjective side effects. Low metabolic stability was observed, as less than 20% of (99m)Tc-HABBN was intact after 30 min. Immunohistochemical staining for GRPR was observed in the prostate cancer specimens in all patients. (99m)Tc-HABBN scintigraphy and SPECT/CT did not detect prostate cancer in patients with proven disease.

CONCLUSIONS:

(99m)Tc-HABBN SPECT/CT for visualization of prostate cancer is safe but hampered by an unexpected low in vivo metabolic stability in man. The difference between the excellent in vitro stability of (99m)Tc-HABBN in human serum samples determined in our previous study regarding (99m)Tc-HABBN and the low in vivo metabolic stability determined in this study, is striking. This issue warrants further study of peptide-based radiopharmaceuticals.

• Authors: Hildo J K Ananias, Zilin Yu, Hilde D Hoving, Stefano Rosati, Rudi A Dierckx, Fan Wang, Yongjun Yan, Xiaoyuan Chen, Jan Pruim, Marjolijn N Lub-de Hooge, Wijnand Helfrich, Philip H Elsinga, Igle J de Jong
• Journal: Nuclear medicine and biology (Nucl Med Biol) Vol. 40 Issue 7 Pg. 933-8 (Oct 2013) ISSN: 1872-9614 [Electronic] United States
• PMID: 23891351 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Organophosphorus Compounds
• Organotechnetium Compounds
• Peptide Fragments
• Receptors, Bombesin
• Sulfonic Acids
• technetium 99m HYNIC-bombesin (7-14)
• triphenylphosphine-3,3',3''-trisulfonic acid
• Glycine
• Aminocaproic Acid
• tricine

Topics

• Aged
• Aminocaproic Acid (chemistry)
• Drug Stability
• Feasibility Studies
• Glycine (analogs & derivatives, chemistry)
• Humans
• Male
• Middle Aged
• Multimodal Imaging
• Organophosphorus Compounds (chemistry)
• Organotechnetium Compounds (blood, chemistry, metabolism)
• Peptide Fragments (blood, chemistry, metabolism)
• Pilot Projects
• Prostatic Neoplasms (diagnosis, diagnostic imaging, metabolism)
• Quality Control
• Receptors, Bombesin (metabolism)
• Sulfonic Acids (chemistry)
• Tomography, Emission-Computed, Single-Photon (methods)
• Tomography, X-Ray Computed (methods)