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Multifunctional dendrimer-based nanoparticles for in vivo MR/CT dual-modal molecular imaging of breast cancer.

Abstract
Development of dual-mode or multi-mode imaging contrast agents is important for accurate and self-confirmatory diagnosis of cancer. We report a new multifunctional, dendrimer-based gold nanoparticle (AuNP) as a dual-modality contrast agent for magnetic resonance (MR)/computed tomography (CT) imaging of breast cancer cells in vitro and in vivo. In this study, amine-terminated generation 5 poly(amidoamine) dendrimers modified with gadolinium chelate (DOTA-NHS) and polyethylene glycol monomethyl ether were used as templates to synthesize AuNPs, followed by Gd(III) chelation and acetylation of the remaining dendrimer terminal amine groups; multifunctional dendrimer-entrapped AuNPs (Gd-Au DENPs) were formed. The formed Gd-Au DENPs were used for both in vitro and in vivo MR/CT imaging of human MCF-7 cancer cells. Both MR and CT images demonstrate that MCF-7 cells and the xenograft tumor model can be effectively imaged. The Gd-Au DENPs uptake, mainly in the cell cytoplasm, was confirmed by transmission electron microscopy. The cell cytotoxicity assay, cell morphology observation, and flow cytometry show that the developed Gd-Au DENPs have good biocompatibility in the given concentration range. Our results clearly suggest that the synthetic Gd-Au DENPs are amenable for dual-modality MR/CT imaging of breast cancer cells.
AuthorsKangan Li, Shihui Wen, Andrew C Larson, Mingwu Shen, Zhuoli Zhang, Qian Chen, Xiangyang Shi, Guixiang Zhang
JournalInternational journal of nanomedicine (Int J Nanomedicine) Vol. 8 Pg. 2589-600 ( 2013) ISSN: 1178-2013 [Electronic] New Zealand
PMID23888113 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Contrast Media
  • DOTA-NHS
  • Dendrimers
  • Succinimides
Topics
  • Analysis of Variance
  • Animals
  • Breast Neoplasms (metabolism, pathology)
  • Cell Survival (drug effects)
  • Contrast Media (chemistry, pharmacokinetics, toxicity)
  • Dendrimers (chemistry, pharmacokinetics, toxicity)
  • Female
  • Heterografts
  • Humans
  • MCF-7 Cells
  • Magnetic Resonance Imaging (methods)
  • Male
  • Mice
  • Mice, Nude
  • Molecular Imaging
  • Nanoparticles (chemistry, toxicity)
  • Succinimides (chemistry)
  • Tissue Distribution
  • Tomography, X-Ray Computed (methods)

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