Abstract |
Development of dual-mode or multi-mode imaging contrast agents is important for accurate and self-confirmatory diagnosis of cancer. We report a new multifunctional, dendrimer-based gold nanoparticle (AuNP) as a dual-modality contrast agent for magnetic resonance (MR)/computed tomography (CT) imaging of breast cancer cells in vitro and in vivo. In this study, amine-terminated generation 5 poly(amidoamine) dendrimers modified with gadolinium chelate ( DOTA-NHS) and polyethylene glycol monomethyl ether were used as templates to synthesize AuNPs, followed by Gd(III) chelation and acetylation of the remaining dendrimer terminal amine groups; multifunctional dendrimer-entrapped AuNPs (Gd-Au DENPs) were formed. The formed Gd-Au DENPs were used for both in vitro and in vivo MR/CT imaging of human MCF-7 cancer cells. Both MR and CT images demonstrate that MCF-7 cells and the xenograft tumor model can be effectively imaged. The Gd-Au DENPs uptake, mainly in the cell cytoplasm, was confirmed by transmission electron microscopy. The cell cytotoxicity assay, cell morphology observation, and flow cytometry show that the developed Gd-Au DENPs have good biocompatibility in the given concentration range. Our results clearly suggest that the synthetic Gd-Au DENPs are amenable for dual-modality MR/CT imaging of breast cancer cells.
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Authors | Kangan Li, Shihui Wen, Andrew C Larson, Mingwu Shen, Zhuoli Zhang, Qian Chen, Xiangyang Shi, Guixiang Zhang |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 8
Pg. 2589-600
( 2013)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 23888113
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Contrast Media
- DOTA-NHS
- Dendrimers
- Succinimides
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Topics |
- Analysis of Variance
- Animals
- Breast Neoplasms
(metabolism, pathology)
- Cell Survival
(drug effects)
- Contrast Media
(chemistry, pharmacokinetics, toxicity)
- Dendrimers
(chemistry, pharmacokinetics, toxicity)
- Female
- Heterografts
- Humans
- MCF-7 Cells
- Magnetic Resonance Imaging
(methods)
- Male
- Mice
- Mice, Nude
- Molecular Imaging
- Nanoparticles
(chemistry, toxicity)
- Succinimides
(chemistry)
- Tissue Distribution
- Tomography, X-Ray Computed
(methods)
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