Antioxidative and anti-inflammatory activities of paeoniflorin and oxypaeoniflora on AGEs-induced mesangial cell damage.

Abstract

Paeonia suffruticosa, an important traditional herbal medicine, has been reported to prevent the pathogenesis of diabetic nephropathy through modulating advanced glycation end products-induced inflammatory and oxidative stress responses. However, little was known about the protective effect of the two major compounds in P. suffruticosa, paeoniflorin and oxypaeoniflora, on advanced glycation end products-induced mesangial cell damage. In the present study, we investigated the protective activities of paeoniflorin and oxypaeoniflora on advanced glycation end product-induced oxidative stress and inflammation in mesangial cells HBZY-1. The IC50 values of paeoniflorin and oxypaeoniflora for inhibiting 2,2'-azinobis-(3-thylbenzothiazoline-6-sulfonic acid) formation were 4.197 × 10-4 M and 1.002 × 10-4 M, respectively. The pretreatment with paeoniflorin and oxypaeoniflora (10-8-10-4 M) significantly increased advanced glycation end product-induced glutathione peroxidase and catalase activities. In the coculture system of HBZY-1 and macrophages, paeoniflorin and oxypaeoniflora could inhibit remarkably the migration of macrophages. Furthermore, paeniflorin and oxypaeniflora attenuated markedly advanced glycation end products-induced inflammation cytokines interleukin-6 and monocyte chemoattractant protein-1 levels in ELISA and western blot analysis in a dose-dependent manner. Taken together, our data provided the reliable evidence that paeniflorin and oxypaeniflora were able to attenuate advanced glycation end products-induced oxidative damage and inflammation in mesangial cells. Paeniflorin and oxypaeniflora might therefore have a beneficial effect in the treatment of diabetic nephropathy.

• Authors: Ming-hua Zhang, Liang Feng, Mao-mao Zhu, Jun-fei Gu, Chan Wu, Xiao-bin Jia
• Journal: Planta medica (Planta Med) Vol. 79 Issue 14 Pg. 1319-23 (Sep 2013) ISSN: 1439-0221 [Electronic] Germany
• PMID: 23881455 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Benzoates
• Bridged-Ring Compounds
• Chemokine CCL2
• Drugs, Chinese Herbal
• Glucosides
• Glycation End Products, Advanced
• Interleukin-6
• Monoterpenes
• peoniflorin
• oxypaeoniflora
• Catalase
• Glutathione Peroxidase

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology, therapeutic use)
• Antioxidants (isolation & purification, metabolism, pharmacology, therapeutic use)
• Benzoates (isolation & purification, pharmacology, therapeutic use)
• Bridged-Ring Compounds (isolation & purification, pharmacology, therapeutic use)
• Catalase (metabolism)
• Chemokine CCL2 (metabolism)
• Diabetic Nephropathies (drug therapy, metabolism, pathology)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (isolation & purification, pharmacology, therapeutic use)
• Glucosides (isolation & purification, pharmacology, therapeutic use)
• Glutathione Peroxidase (metabolism)
• Glycation End Products, Advanced (metabolism)
• Inflammation (drug therapy, metabolism)
• Inhibitory Concentration 50
• Interleukin-6 (metabolism)
• Macrophages (drug effects)
• Mesangial Cells (drug effects, metabolism, pathology)
• Monoterpenes
• Oxidative Stress (drug effects)
• Paeonia (chemistry)
• Phytotherapy
• Rats