Abstract | OBJECTIVES: METHODS: In vivo experiments were conducted on 102 Wistar Han rats. The experiments investigated the hematotoxic and oxidative stress effects of hydroxylamine sulfate in acute and subacute toxicity treatment of animals. Electron Spin Resonance was used for quantitative determination of blood and liver tissue parameters alterations after intoxication. The osmotic fragility of erythrocytes, lipid peroxidation intensity and level of SH-groups in liver of rats were determined by established biochemical assays. RESULTS:
Hydroxylamine sulfate cause an acute hematotoxicity and oxidative stress in vivo as demonstrated by the appearance of free oxidized iron in blood, reduced glutathione content and increased lipid peroxidation in liver. The experimental studies showed the formation of Hb-NO, MetHb in erythrocytes and as well of stable complex of reduced iron (Fe(2+)) with hydroxylamine sulfate. Methylene blue treatment does not reduce the Hb-NO or MetHb levels in intoxicated animals while administration of α- tocopherol acetate reduces substantially lipid peroxidation. CONCLUSIONS:
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Authors | Mykola G Prodanchuk, Aristidis M Tsatsakis, Georgiy M Prodanchuk, Andreas K Tsakalof |
Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 61
Pg. 227-32
(Nov 2013)
ISSN: 1873-6351 [Electronic] England |
PMID | 23872126
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Chemical References |
- Hydroxylamines
- alpha-Tocopherol
- Sodium Nitrite
- Methylene Blue
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Topics |
- Animals
- Electron Spin Resonance Spectroscopy
- Erythrocytes
(drug effects)
- Hydroxylamines
(poisoning, toxicity)
- Lipid Peroxidation
(drug effects)
- Liver
(drug effects)
- Methylene Blue
(pharmacology)
- Oxidative Stress
(drug effects)
- Poisoning
(drug therapy)
- Rats
- Rats, Wistar
- Sodium Nitrite
(poisoning)
- Toxicity Tests, Acute
- Toxicity Tests, Subacute
- alpha-Tocopherol
(pharmacology)
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