Abstract | BACKGROUND: In chronic liver disease, hepatic stellate cells (HSCs) are activated, highly proliferative and produce excessive amounts of extracellular matrix, leading to liver fibrosis. Elevated levels of toxic reactive oxygen species (ROS) produced during chronic liver injury have been implicated in this activation process. Therefore, activated hepatic stellate cells need to harbor highly effective anti-oxidants to protect against the toxic effects of ROS. AIM: To investigate the protective mechanisms of activated HSCs against ROS-induced toxicity. METHODS: RESULTS: CONCLUSION: Activated HSCs have increased ROS-detoxifying capacity compared to quiescent HSCs. Glutathione levels increase during HSC activation and protect against ROS-induced necrosis, whereas hydrogen peroxide-detoxifying enzymes protect against apoptotic cell death.
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Authors | Sandra Dunning, Atta Ur Rehman, Marjolein H Tiebosch, Rebekka A Hannivoort, Floris W Haijer, Jannes Woudenberg, Fiona A J van den Heuvel, Manon Buist-Homan, Klaas Nico Faber, Han Moshage |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1832
Issue 12
Pg. 2027-34
(Dec 2013)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 23871839
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013. |
Chemical References |
- Antioxidants
- Oxidants
- RNA, Messenger
- Hydrogen Peroxide
- Catalase
- Glutathione Peroxidase
- Superoxide Dismutase
- superoxide dismutase 2
- Glutathione
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Topics |
- Animals
- Antioxidants
(metabolism)
- Apoptosis
(drug effects)
- Blotting, Western
- Catalase
(genetics, metabolism)
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Fluorescent Antibody Technique
- Glutathione
(metabolism)
- Glutathione Peroxidase
(genetics, metabolism)
- Hepatic Stellate Cells
(drug effects, metabolism, pathology)
- Hydrogen Peroxide
(pharmacology)
- Male
- Necrosis
- Oxidants
(pharmacology)
- Oxidative Stress
(drug effects)
- RNA, Messenger
(genetics)
- Rats
- Rats, Wistar
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Superoxide Dismutase
(genetics, metabolism)
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