The regulation of Toll-like receptor 2 by miR-143 suppresses the invasion and migration of a subset of human colorectal carcinoma cells.

Abstract

BACKGROUND:

The Toll-like receptor 2 (TLR2)-driven tissue response may promote neoangiogenesis and tumour growth by mechanisms that are poorly understood.

METHODS:

We investigated the expression levels of TLR2 and associated-miRNAs in colorectal carcinoma (CRC) tissues and cell lines using real-time PCR, northern blotting and western blotting. Survival curver was generated by Log-Rank test and the role of TLR2 signalling in tumour invasion and migration was determined by transwell analysis kits.

RESULTS:

We observed that the tissues from CRC patients express relatively high levels of TLR2. Targeting TLR2 markedly reduces the invasion and migration of CRC cells. We also found that miR-143, a putative tumour suppressor that is down-regulated in CRC tissues, reduces the invasion and migration of CRC cells primarily via TLR2. Utilising a xenograft mouse model, we demonstrated that re-expression of miR-143 inhibits CRC cell colonisation in vivo.

CONCLUSION:

miR-143 blocks the TLR2 signalling pathway in human CRC cells. This knowledge may pave the way for new clinical applications utilising miR-143 mimics in the treatment of patients with CRC.

• Authors: Haiyan Guo, Ying Chen, Xiaobo Hu, Guanxiang Qian, Shengfang Ge, Jianjun Zhang
• Journal: Molecular cancer (Mol Cancer) Vol. 12 Pg. 77 (Jul 17 2013) ISSN: 1476-4598 [Electronic] England
• PMID: 23866094 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN143 microRNA, human
• MicroRNAs
• Toll-Like Receptor 2

Topics

• Animals
• Base Pairing
• Base Sequence
• Cell Line, Tumor
• Cell Movement (genetics)
• Colorectal Neoplasms (genetics, metabolism, pathology)
• Disease Models, Animal
• Gene Expression
• Humans
• Mice
• MicroRNAs (chemistry, genetics)
• Neoplasm Invasiveness
• Neoplasm Metastasis
• Toll-Like Receptor 2 (genetics, metabolism)
• Transplantation, Heterologous