The dose of cyclophosphamide for treating paraquat-induced rat lung injury.

Abstract

BACKGROUND/AIMS:

Cyclophosphamide (CP) is a promising treatment for severe cases of paraquat (PQ) poisoning. We investigated the effective dose of CP for mitigating PQ-induced lung injury.

METHODS:

Adult male Sprague-Dawley rats were allocated into five groups: control, PQ (35 mg/kg, intraperitoneal injection), and PQ + CP (1.5, 15, or 30 mg/kg). The dimensions of lung lesions were determined using X-ray microtomography (micro-CT), and histological changes and cytokine levels were recorded.

RESULTS:

The micro-CT results showed that 15 mg/kg CP was more effective than 1.5 mg/kg CP for treating PQ-induced lung injury. At a dose of 1.5 mg/kg, CP alleviated the histological evidence of inflammation and altered superoxide dismutase activity. Using 15 mg/kg CP reduced the elevated catalase activity and serum transforming growth factor (TGF)-β1 level.

CONCLUSIONS:

A CP dose of > 15 mg/kg is effective for reducing the severity of PQ-induced lung injury as determined by histological and micro-CT tissue examination, possibly by modulating antioxidant enzyme and TGF-β1 levels.

• Authors: Jae-Sung Choi, Sung-Shick Jou, Mee-Hye Oh, Young-Hee Kim, Min-Ju Park, Hyo-Wook Gil, Ho-Yeon Song, Sae-Yong Hong
• Journal: The Korean journal of internal medicine (Korean J Intern Med) Vol. 28 Issue 4 Pg. 420-7 (Jul 2013) ISSN: 2005-6648 [Electronic] Korea (South)
• PMID: 23864800 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Immunosuppressive Agents
• Inflammation Mediators
• Tgfb1 protein, rat
• Transforming Growth Factor beta1
• Cyclophosphamide
• Catalase
• Superoxide Dismutase
• Paraquat

Topics

• Acute Lung Injury
• Animals
• Catalase (metabolism)
• Cyclophosphamide (pharmacology)
• Cytokines (metabolism)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Immunosuppressive Agents (pharmacology)
• Inflammation Mediators (metabolism)
• Lung (diagnostic imaging, drug effects, metabolism, pathology)
• Lung Injury (chemically induced, diagnosis, drug therapy, metabolism)
• Male
• Oxidative Stress (drug effects)
• Paraquat
• Pulmonary Edema (chemically induced, diagnosis, drug therapy, metabolism)
• Rats
• Rats, Sprague-Dawley
• Severity of Illness Index
• Superoxide Dismutase (metabolism)
• Transforming Growth Factor beta1 (metabolism)
• X-Ray Microtomography