Abstract | BACKGROUND: Several proteins have been proposed as new urinary biomarkers of kidney injuries, but they are not always capable of identifying the kidney nephron segment that has been injured. Since calbindin 1 protein is exclusively localized in the kidney distal nephron segment, it is presumed that its expression is altered during distal nephron segment injuries, resulting in changes in its urinary excretion. METHODS:
Calbindin 1 expression in normal rat kidneys was compared with that in the kidneys of rats that had suffered distal nephron segment injuries (unilateral ureteral obstruction [UUO] or anti-glomerular basement membrane glomerulonephritis [ anti-GBM GN]) using immunohistochemical examinations and real-time polymerase chain reaction. The urinary calbindin 1 protein concentration of normal rats was also compared with that of anti-GBM GN rats and of cisplatin nephropathy rats using Western blotting. We also compared the kidney and urinary calbindin 1 protein concentrations of normal human subjects with those of proteinuric patients [ immunoglobulin (Ig)A nephropathy; IgAN] with distal nephron segment injuries. RESULTS: CONCLUSION:
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Authors | Tomoko Iida, Hidehiko Fujinaka, Bo Xu, Ying Zhang, Sameh Magdeldin, Masaaki Nameta, Zan Liu, Yutaka Yoshida, Eishin Yaoita, Shuichi Tomizawa, Akihiko Saito, Tadashi Yamamoto |
Journal | Clinical and experimental nephrology
(Clin Exp Nephrol)
Vol. 18
Issue 3
Pg. 432-43
(Jun 2014)
ISSN: 1437-7799 [Electronic] Japan |
PMID | 23864347
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- CALB1 protein, human
- Calb1 protein, rat
- Calbindin 1
- RNA, Messenger
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Topics |
- Adolescent
- Animals
- Biomarkers
(metabolism, urine)
- Calbindin 1
(metabolism, urine)
- Child
- Disease Models, Animal
- Glomerulonephritis
(urine)
- Glomerulonephritis, IGA
(urine)
- Humans
- Kidney
(metabolism, pathology)
- Kidney Tubules, Distal
(metabolism, pathology, physiopathology)
- Male
- Nephrons
(metabolism, pathology, physiopathology)
- Proteinuria
(urine)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Inbred WKY
- Ureteral Obstruction
(urine)
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