Anti-metastatic and differential effects on protein expression of epigallocatechin-3-gallate in HCCLM6 hepatocellular carcinoma cells.

Abstract

Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and the third highest cause of cancer-related mortality in humans. Epigallocatechin-3-gallate (EGCG) has been shown to inhibit the metastatic activity of certain cancer cells. The aim of this study was to determine the effects and molecular mechanism(s) of action of EGCG in human HCC cells. A migration and invasion assay for the metastatic behavior of HCCLM6 cells was performed. The anti-metastatic effects of EGCG were investigated by RT-PCR and gelatin zymography. A total cellular protein profile was obtained using 2-dimensional gel electrophoresis (2-DE), followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) analyses of proteins with significant differences in expression following treatment with EGCG. The results revealed that EGCG induced apoptosis and inhibited the metastasis of HCCLM6 cells. The anti-metastatic effects of EGCG were associated with the inhibition of matrix metalloproteinase (MMP)-2 and MMP-9 activity. The expression levels of far upstream element (FUSE) binding protein 1 (FUBP1), heat shock protein beta 1 (HSPB1), heat shock 60 kDa protein 1 (chaperonin) (CH60) and nucleophosmin (NPM) proteins, which are associated with metastasis, were significantly altered in the EGCG-treated HCCLM6 cells. The data from the present study suggest that EGCG has potential as a therapeutic agent for the treatment of HCC.

• Authors: Yunjuan Zhang, Lawrence Owusu, Wei Duan, Tao Jiang, Shizhu Zang, Ayaz Ahmed, Yi Xin
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 32 Issue 4 Pg. 959-64 (Oct 2013) ISSN: 1791-244X [Electronic] Greece
• PMID: 23863984 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• DNA-Binding Proteins
• FUBP1 protein, human
• HSP27 Heat-Shock Proteins
• HSPB1 protein, human
• Heat-Shock Proteins
• Molecular Chaperones
• NPM1 protein, human
• Nuclear Proteins
• RNA-Binding Proteins
• STIP1 protein, human
• Nucleophosmin
• Catechin
• epigallocatechin gallate
• MMP2 protein, human
• Matrix Metalloproteinase 2
• MMP9 protein, human
• Matrix Metalloproteinase 9
• DNA Helicases

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (metabolism)
• Catechin (analogs & derivatives, pharmacology)
• Cell Differentiation (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• DNA Helicases (genetics, metabolism)
• DNA-Binding Proteins (genetics, metabolism)
• HSP27 Heat-Shock Proteins (genetics, metabolism)
• Heat-Shock Proteins (genetics, metabolism)
• Humans
• Liver Neoplasms (metabolism)
• Matrix Metalloproteinase 2 (genetics, metabolism)
• Matrix Metalloproteinase 9 (genetics, metabolism)
• Molecular Chaperones
• Neoplasm Metastasis (drug therapy)
• Nuclear Proteins (genetics, metabolism)
• Nucleophosmin
• Protein Processing, Post-Translational (genetics)
• Proteomics
• RNA-Binding Proteins