Magnesium lithospermate B reduces myocardial ischemia/reperfusion injury in rats via regulating the inflammation response.

Abstract

CONTEXT:

Magnesium lithospermate B (MLB), an active polyphenol acid of Danshen [Radix Salviae miltiorrhizae (Labiatae)], showed renoprotective, neuroprotective and myocardial salvage effects. Previous studies demonstrated that MLB could effectively suppress the production of cytokines and their associated signaling pathways in activated human T cells.

OBJECTIVE:

The purpose of this study was to examine the beneficial effects of MLB on myocardial ischemia/reperfusion (MI/R) injury and to explore its potential mechanisms related to anti-inflammation.

MATERIALS AND METHODS:

Sprague-Dawley rats were grouped as sham group, model group and MLB-treated (15, 30 and 60 mg/kg) groups. Animals were subjected to MI/R injury by the occlusion of left anterior descending artery for 30 min followed by reperfusion for 3 h. At the end of reperfusion, blood samples were collected to determine the serum levels of cardiac troponin (cTnI), creatine kinase-MB (CK-MB), tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6). Hearts were harvested to assess infarct size, histopathological changes and the activity of myeloperoxidase (MPO). The expression of phosphor-IkB-α and phosphor-nuclear factor kappa B (NF-κB) were assayed by western blot.

RESULTS:

MLB administration significantly (p < 0.05) reduced: (1) ST-segment elevation (0.23 mv), (2) the infarct size (22.5%), (3) histological scores of myocardial injury (1.67 score), (4) myocardial injury marker enzymes: cTnI (5.64 ng/ml) and CK-MB (49.57 ng/ml) levels, (5) pro-inflammatory cytokines: TNF-α (97.36 pg/ml), IL-1β (93.35 pg/ml) and IL-6 (96.84 pg/ml) levels, (6) MPO activity (1.82 U/mg), (7) phosphor-NF-κB (0.87) and phosphor-IkB-α (0.96) expression.

DISCUSSION AND CONCLUSION:

Our study provided evidence that MLB ameliorated the inflammatory process associated with MI/R injury via NF-κB inactivation.

• Authors: Wei Quan, Guo Wei, Dan Zhou, Yanrong Zhu, Chao Guo, Yanhua Wang, Yan Weng, Miaomiao Xi, Aidong Wen
• Journal: Pharmaceutical biology (Pharm Biol) Vol. 51 Issue 11 Pg. 1355-62 (Nov 2013) ISSN: 1744-5116 [Electronic] England
• PMID: 23863120 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Biomarkers
• Cytokines
• Drugs, Chinese Herbal
• I-kappa B Proteins
• Inflammation Mediators
• NF-kappa B
• NFKBIA protein, human
• Nfkbia protein, rat
• Troponin I
• lithospermate B
• NF-KappaB Inhibitor alpha
• Peroxidase
• Creatine Kinase, MB Form

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Biomarkers (blood)
• Creatine Kinase, MB Form (blood)
• Cytokines (blood)
• Disease Models, Animal
• Drugs, Chinese Herbal (pharmacology)
• Electrocardiography
• I-kappa B Proteins (metabolism)
• Inflammation (blood, immunology, prevention & control)
• Inflammation Mediators (blood)
• Male
• Myocardial Infarction (blood, immunology, pathology, prevention & control)
• Myocardial Reperfusion Injury (blood, immunology, pathology, prevention & control)
• Myocardium (immunology, metabolism, pathology)
• NF-KappaB Inhibitor alpha
• NF-kappa B (metabolism)
• Peroxidase (metabolism)
• Phosphorylation
• Rats
• Rats, Sprague-Dawley
• Signal Transduction (drug effects)
• Time Factors
• Troponin I (blood)