The antimicrobial agent C31G is effective for therapy for HSV-1 ocular keratitis in the rabbit eye model.

Abstract

The amphoteric C31G solution contains equimolar alkyl dimethlyglycine and alkyl dimethyl amine oxide buffered with citric acid. C31G acts as a broad spectrum antiviral and an antibacterial. No previous in vivo studies have been done to test C31G in an animal model of HSV-1 ocular keratitis. We assessed the anti-herpetic activity of C31G in the rabbit eye model using three treatment groups: (1) 1% trifluorothymidine (TFT); (2) 0.25% C31G plus 0.5% hydroxypropyl methylcellulose (HPMC); and (3) vehicle, 0.5% HPMC. Scarified rabbit corneas were inoculated with the HSV-1 strain McKrae. On post inoculation (PI) day 3, rabbits were placed in three balanced groups based on slit-lamp examination (SLE) scores. Treatment began on PI day 3, five times a day for five consecutive days. In addition to the daily, masked SLE scoring, the eyes were assessed daily for stromal opacity, scleral inflammation, neovascularization, eyelid inflammation, inflammatory discharge, and epiphora. C31G and TFT were very effective in reducing the lesions and pathogenesis associated with HSV-1 ocular keratitis. The vehicle control scores were significantly higher and did not effectively treat HSV-1 keratitis. C31G has the potential to be used to treat herpetic keratitis as well as other herpetic topical lesions in humans.

• Authors: James M Hill, Ethan M Stern, Partha S Bhattacharjee, Daniel Malamud, Christian Clement, Paulo Rodriguez, Walter J Lukiw, Augusto C Ochoa, Timothy P Foster, Cruz Velasco, Harris E McFerrin Jr
• Journal: Antiviral research (Antiviral Res) Vol. 100 Issue 1 Pg. 14-9 (Oct 2013) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 23860013 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013. Published by Elsevier B.V.

Chemical References

• Antiviral Agents
• Fatty Acids, Unsaturated
• Betaine
• C 31G

Topics

• Animals
• Antiviral Agents (administration & dosage)
• Betaine (administration & dosage, analogs & derivatives)
• Cornea (pathology, virology)
• Disease Models, Animal
• Fatty Acids, Unsaturated (administration & dosage)
• Herpesvirus 1, Human (drug effects, physiology)
• Humans
• Keratitis, Herpetic (drug therapy, pathology, virology)
• Rabbits