Influence of sleep deprivation on expression of MKK4 and c-fos in the mandibular condylar cartilage of rats.

The aim of this study was to investigate the changes in expression of mitogen-activated protein kinase kinase 4 (MKK4) and c-fos in the mandibular condylar cartilage of rats that had been subjected to sleep deprivation. One hundred and twenty female Wistar rats were randomly divided into 6 groups with 20 in each: sleep deprivation for 2 days, 4 days, 6 days, and 8 days, large-platform controls, and cage controls. After sleep deprivation by the modified multiple platform method the sleep-deprived rats were killed. The large-platform and cage control rats were killed at the same time as the rats deprived of sleep for 8 days. Haematoxylin and eosin were used to record the morphological changes in cartilage, and immunohistochemistry and real-time quantitative polymerase chain reaction (PCR) were used to detect the expression of MKK4 and c-fos. Pathological alterations were apparent after 6 and 8 days of sleep deprivation. Compared with control groups, the expression of MKK4 in the sleep-deprived groups was lower, while that of c-fos was higher. As the duration of sleep deprivation increased, the expression of MKK4 decreased. These results indicate that the variation in expression of MKK4 and c-fos may be correlated with pathological changes induced by sleep deprivation in mandibular condylar cartilage in rats.
AuthorsJinlong Chen, Gaoyi Wu, Guoxiong Zhu, Peihuan Wang, Hongyu Chen, Huaqiang Zhao
JournalThe British journal of oral & maxillofacial surgery (Br J Oral Maxillofac Surg) Vol. 51 Issue 8 Pg. e250-5 (Dec 2013) ISSN: 1532-1940 [Electronic] Scotland
PMID23859770 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
Chemical References
  • Coloring Agents
  • Fluorescent Dyes
  • Proto-Oncogene Proteins c-fos
  • MAP Kinase Kinase 4
  • Animals
  • Cartilage, Articular (enzymology, metabolism, pathology)
  • Chondrocytes (enzymology, metabolism, pathology)
  • Coloring Agents
  • Disease Models, Animal
  • Female
  • Fluorescent Dyes
  • Immunohistochemistry
  • MAP Kinase Kinase 4 (analysis)
  • Mandibular Condyle (enzymology, metabolism, pathology)
  • Proto-Oncogene Proteins c-fos (analysis)
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction
  • Sleep Deprivation (enzymology, metabolism)
  • Temporomandibular Joint (enzymology, metabolism, pathology)
  • Time Factors

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