Influence of sleep deprivation on expression of MKK4 and c-fos in the mandibular condylar cartilage of rats.

Abstract

The aim of this study was to investigate the changes in expression of mitogen-activated protein kinase kinase 4 (MKK4) and c-fos in the mandibular condylar cartilage of rats that had been subjected to sleep deprivation. One hundred and twenty female Wistar rats were randomly divided into 6 groups with 20 in each: sleep deprivation for 2 days, 4 days, 6 days, and 8 days, large-platform controls, and cage controls. After sleep deprivation by the modified multiple platform method the sleep-deprived rats were killed. The large-platform and cage control rats were killed at the same time as the rats deprived of sleep for 8 days. Haematoxylin and eosin were used to record the morphological changes in cartilage, and immunohistochemistry and real-time quantitative polymerase chain reaction (PCR) were used to detect the expression of MKK4 and c-fos. Pathological alterations were apparent after 6 and 8 days of sleep deprivation. Compared with control groups, the expression of MKK4 in the sleep-deprived groups was lower, while that of c-fos was higher. As the duration of sleep deprivation increased, the expression of MKK4 decreased. These results indicate that the variation in expression of MKK4 and c-fos may be correlated with pathological changes induced by sleep deprivation in mandibular condylar cartilage in rats.

• Authors: Jinlong Chen, Gaoyi Wu, Guoxiong Zhu, Peihuan Wang, Hongyu Chen, Huaqiang Zhao
• Journal: The British journal of oral & maxillofacial surgery (Br J Oral Maxillofac Surg) Vol. 51 Issue 8 Pg. e250-5 (Dec 2013) ISSN: 1532-1940 [Electronic] Scotland
• PMID: 23859770 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Coloring Agents
• Fluorescent Dyes
• Proto-Oncogene Proteins c-fos
• MAP Kinase Kinase 4

Topics

• Animals
• Cartilage, Articular (enzymology, metabolism, pathology)
• Chondrocytes (enzymology, metabolism, pathology)
• Coloring Agents
• Disease Models, Animal
• Female
• Fluorescent Dyes
• Immunohistochemistry
• MAP Kinase Kinase 4 (analysis)
• Mandibular Condyle (enzymology, metabolism, pathology)
• Proto-Oncogene Proteins c-fos (analysis)
• Random Allocation
• Rats
• Rats, Wistar
• Real-Time Polymerase Chain Reaction
• Sleep Deprivation (enzymology, metabolism)
• Temporomandibular Joint (enzymology, metabolism, pathology)
• Time Factors