Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus.

Abstract

Nicotiana tabacum cv. Xanthi nn plants were transformed with nucleotides 3472-4916 of tobacco mosaic virus (TMV) strain U1. This sequence contains all but the three 3 terminal nucleotides of the TMV 54-kDa gene, which encodes a putative component of the replicase complex. These plants were resistant to infection when challenged with either TMV U1 virions or TMV U1 RNA at concentrations of up to 500 micrograms/ml or 300 micrograms/ml, respectively, the highest concentrations tested. Resistance was also exhibited when plants were inoculated at 100 micrograms/ml with the closely related TMV mutant YSI/1 but was not shown in plants challenged at the same concentrations with the more distantly related TMV strains U2 or L or cucumber mosaic virus. Although the copy number of the 54-kDa gene sequence varied in individual transformants from 1 to approximately 5, the level of resistance in plants was not dependent on the number of copies of the 54-kDa gene sequence integrated. The transformed plants accumulated a 54-kDa gene sequence-specific RNA transcript of the expected size, but no protein product was detected.

• Authors: D B Golemboski, G P Lomonossoff, M Zaitlin
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 87 Issue 16 Pg. 6311-5 (Aug 1990) ISSN: 0027-8424 [Print] United States
• PMID: 2385595 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA, Viral
• RNA, Viral

Topics

• Base Sequence
• Cloning, Molecular
• DNA, Viral (genetics)
• Genes, Viral
• Immunity, Innate (genetics)
• Molecular Sequence Data
• Plants, Toxic
• Plasmids
• RNA, Viral (genetics, isolation & purification)
• Restriction Mapping
• Tobacco (genetics, microbiology)
• Tobacco Mosaic Virus (genetics, pathogenicity)
• Transformation, Genetic