A comparative study of PGI2 mimetics used clinically on the vasorelaxation of human pulmonary arteries and veins, role of the DP-receptor.

Abstract

Prostacyclin (PGI2) and its mimetics (iloprost, treprostinil, beraprost and MRE-269) are potent vasodilators (via IP-receptor activation) and a major therapeutic intervention for pulmonary hypertension (PH). These PGI2 mimetics have anti-proliferative and potent vasodilator effects on pulmonary vessels. We compared the relaxant effects induced by these recognized IP-agonists in isolated human pulmonary arteries (HPA) and veins (HPV). In addition, using selective antagonists, the possible activation of other prostanoid relaxant receptors (DP, EP4) was investigated. Iloprost and treprostinil were the more potent relaxant agonists when both vessels were analyzed. HPA were significantly more sensitive to iloprost than to treprostinil, pEC50 values: 7.94±0.06 (n=23) and 6.73±0.08 (n=33), respectively. In contrast, in HPV these agonists were equipotent. The relaxations induced by treprostinil were completely or partially inhibited by IP-antagonists in HPA or HPV, respectively. The effects of the IP-agonists were not significantly modified by the EP4 antagonist. Finally, DP-antagonists inhibited the relaxations induced by treprostinil in HPV, suggesting that the DP-receptor plays a role in treprostinil-induced relaxation in the HPV. These data suggest that iloprost and treprostinil should be the most effective clinically available agonists to decrease pulmonary vascular resistance and to prevent oedema formation (by similar decrease in HPA and HPV resistance) in PH patients.

• Authors: Chabha Benyahia, Kamel Boukais, Ingrid Gomez, Adam Silverstein, Lucie Clapp, Aurélie Fabre, Claire Danel, Guy Leséche, Dan Longrois, Xavier Norel
• Journal: Prostaglandins & other lipid mediators (Prostaglandins Other Lipid Mediat) Vol. 107 Pg. 48-55 (Dec 2013) ISSN: 1098-8823 [Print] United States
• PMID: 23850788 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Acetates
• PTGIR protein, human
• Pyrazines
• Receptors, Epoprostenol
• Receptors, Immunologic
• Receptors, Prostaglandin
• Receptors, Prostaglandin E, EP4 Subtype
• Vasodilator Agents
• beraprost
• Epoprostenol
• (4-((5,6-diphenylpyrazin-2-yl)(isopropyl)amino)butoxy)acetic acid
• Iloprost
• treprostinil
• prostaglandin D2 receptor

Topics

• Acetates (pharmacology)
• Aged
• Drug Evaluation, Preclinical
• Epoprostenol (analogs & derivatives, pharmacology)
• Female
• Humans
• Iloprost (pharmacology)
• In Vitro Techniques
• Inhibitory Concentration 50
• Male
• Middle Aged
• Molecular Mimicry
• Pulmonary Artery (drug effects, physiology)
• Pulmonary Veins (drug effects, physiology)
• Pyrazines (pharmacology)
• Receptors, Epoprostenol
• Receptors, Immunologic (antagonists & inhibitors, metabolism)
• Receptors, Prostaglandin (antagonists & inhibitors, metabolism)
• Receptors, Prostaglandin E, EP4 Subtype (antagonists & inhibitors, metabolism)
• Vasodilation
• Vasodilator Agents (pharmacology)