Abstract |
In order to study localization of metastatic tumors with a radiolabeled monoclonal antibody, a pulmonary metastases model was devised in athymic mice. Metastatic pulmonary sarcoma colonies were verified by histological examination. A murine monoclonal antibody (MAb 19-24) directed against a human sarcoma antigen was labeled with indium-111 (111In) by use of the linker 1-(p-isothiocyanatobenzyl)-diethylenetriaminepentaacetic acid ( SCN-Bz-DTPA). MAb P3 was similarly labeled as a negative control. In the group given MAb 19-24, the percent injected dose per gram lung tissue bearing tumor colonies (30.1%, 29.6%, and 27.7% on Days 1, 2, and 3, respectively) was significantly (p less than 0.05) higher than in those receiving MAb P3. Hepatic activities of both 111In-MAb 19-24 and 111In-MAb P3 were low. The lungs with tumor colonies demonstrated clearest images on Day 3. The specific binding of 111In-SCN-Bz-DTPA-labeled MAb 19-24 to pulmonary xenografts without appreciable liver uptake indicates that it may be useful in the clinical localization of pulmonic metastatic lesions.
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Authors | J A Greager, T C Chao, M J Blend, R W Atcher, O A Gansow, M W Brechbiel, T K Das Gupta |
Journal | Journal of nuclear medicine : official publication, Society of Nuclear Medicine
(J Nucl Med)
Vol. 31
Issue 8
Pg. 1378-83
(Aug 1990)
ISSN: 0161-5505 [Print] United States |
PMID | 2384807
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Indium Radioisotopes
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Topics |
- Animals
- Antibodies, Monoclonal
(pharmacokinetics)
- Fibrosarcoma
(diagnostic imaging, secondary)
- Humans
- Indium Radioisotopes
(pharmacokinetics)
- Lung Neoplasms
(diagnostic imaging, secondary)
- Male
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Radionuclide Imaging
- Tissue Distribution
- Transplantation, Heterologous
- Tumor Cells, Cultured
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