Quantification of urinary 18-hydroxycortisol using LC-MS/MS.

Abstract

BACKGROUND:

Urinary 18-hydroxycortisol has been investigated as a marker of aldosterone-producing adenoma (APA). The aim of this study was to develop and validate a method for the measurement of 18-hydroxycortisol using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

METHODS:

Urine was collected over a 24-hour period in patients with APA (n = 11), idiopathic hyperaldosteronism (IHA, n = 9), and essential hypertension (EH, n = 6). 18-Hydroxycortisol was extracted in solid-phase, and measured by LC-MS/MS based on selected reaction monitoring.

RESULTS:

The method allowed quantification of 18-hydroxycortisol with a lower quantification limit of 0.26 nmol/L, intra- and inter-assay coefficients of variation of <3.4% and a range of analytical recovery of 98.0-103.7%. Urinary 18-hydroxycortisol excretion for APA, IHA and EH were determined as 725 (SD 451), 102 (SD 68) and 88 (SD 76) nmol/day, respectively.

CONCLUSIONS:

The proposed method met the basic analytical requirements and was considered to be useful in the screening and differential diagnosis of APA.

• Authors: Shigeki Jin, Norio Wada, Yuji Takahashi, Shu-Ping Hui, Toshihiro Sakurai, Hirotoshi Fuda, Seiji Takeda, Masato Fujikawa, Katsuyuki Yanagisawa, Shigeo Ikegawa, Takao Kurosawa, Hitoshi Chiba
• Journal: Annals of clinical biochemistry (Ann Clin Biochem) Vol. 50 Issue Pt 5 Pg. 450-6 (Sep 2013) ISSN: 1758-1001 [Electronic] England
• PMID: 23847032 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Aldosterone
• 18-hydroxycortisol
• Hydrocortisone

Topics

• Adenoma (urine)
• Adrenal Gland Neoplasms (urine)
• Aldosterone (biosynthesis)
• Biomarkers, Tumor (urine)
• Chromatography, Liquid
• Essential Hypertension
• Humans
• Hydrocortisone (analogs & derivatives, urine)
• Hyperaldosteronism (urine)
• Hypertension (urine)
• Limit of Detection
• Reproducibility of Results
• Solid Phase Microextraction
• Tandem Mass Spectrometry